Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy

Cytotherapy
Mirelle J A J HuijskensWilfred T V Germeraad

Abstract

Natural killer (NK) cell-based immunotherapy is a promising treatment for a variety of malignancies. However, generating sufficient cell numbers for therapy remains a challenge. To achieve this, optimization of protocols is required. Mature NK cells were expanded from peripheral blood mononuclear cells PBMCs in the presence of anti-CD3 monoclonal antibody and interleukin-2. Additionally, NK-cell progenitors were generated from CD34(+) hematopoietic stem cells or different T/NK-cell progenitor populations. Generated NK cells were extensively phenotyped, and functionality was determined by means of cytotoxicity assay. Addition of ascorbic acid (AA) resulted in more proliferation of NK cells without influencing NK-cell functionality. In more detail, PBMC-derived NK cells expanded 2362-fold (median, range: 90-31,351) in the presence of AA and were capable of killing tumor cells under normoxia and hypoxia. Moreover, hematopoietic stem cell-derived progenitors appeared to mature faster in the presence of AA, which was also observed in the NK-cell differentiation from early T/NK-cell progenitors. Mature NK cells proliferate faster in the presence of phospho-L-AA, resulting in higher cell numbers with accurate functional capacity, whic...Continue Reading

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Citations

Mar 26, 2016·Results in Immunology·Mirelle J A J HuijskensGerard M J Bos
Dec 8, 2016·Revista da Associação Médica Brasileira·Ana Maria Oliveira Ferreira da MataBenedito Borges da Silva
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Feb 9, 2020·Journal of Leukocyte Biology·Christian PetersDieter Kabelitz
Oct 31, 2019·European Journal of Microbiology & Immunology·Soraya MousaviMarkus M Heimesaat
Dec 30, 2017·Experimental and Therapeutic Medicine·Huiying YuDongchu Ma
Jul 7, 2020·International Journal of Molecular Medicine·Felician StancioiuAristidis Tsatsakis
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Mar 15, 2018·Antioxidants·Gwendolyn N Y van GorkomGerard M J Bos
Jun 7, 2019·Cellular & Molecular Immunology·Léonce KouakanouDieter Kabelitz
Jan 24, 2019·Journal of Immunology Research·Malgorzata Grudzien, Andrzej Rapak
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Oct 13, 2020·Pharmacology & Therapeutics·Laura MasuelliRoberto Bei
Jan 8, 2021·Journal of Hematology & Oncology·Sizhe LiuJennifer Wu
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Dec 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Takwa BedhiafiSaid Dermime

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