Ascorbic acid suppresses cell death in rat DS-sarcoma cancer cells induced by 5-aminolevulinic acid-based photodynamic therapy

Free Radical Biology & Medicine
Juergen FrankHans Konrad Biesalski

Abstract

Especially in the public, vitamin C is considered supportive for the treatment of cancer and supplementation is common. However, the underlying mechanism that most chemotherapeutic agents, ionizing radiation, and photodynamic therapy exert on tumor cell kill is an increased production of reactive oxygen species (ROS) leading to irreversible tissue injury. Therefore, antioxidants like ascorbic acid (AA) may prevent cancer cells of cellular free radical damage and may therefore be contraindicated in patients undergoing tumor treatment. We report on the effects of AA on markers of oxidative stress and apoptosis in rat DS-sarcoma cells on 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT). AA dose-dependently protected cancer cells against lipid and protein oxidation caused by ALA-PDT treatment. By real-time RT-PCR analysis an impressive increase of FasL (124-fold) and TNF-alpha (121-fold) mRNA was detected after PDT treatment. In addition, a decrease in mitochondrial transmembrane potential followed by the mitochondrial release of apoptosis-inducing factor (AIF) was observed. All these early signs of apoptosis were significantly reduced by AA, resulting in a 2.1-fold increased cell survival rate on ALA-PDT treatment. In co...Continue Reading

References

Mar 1, 1977·Irish Journal of Medical Science·M J MoriartyM K O'Connor
Jun 15, 1989·International Journal of Cancer. Journal International Du Cancer·H LangemannC G Honegger
Aug 1, 1994·Journal of Bioenergetics and Biomembranes·W W Wells, D P Xu
Sep 1, 1993·Canadian Journal of Physiology and Pharmacology·A C Chan
Jan 1, 1996·Free Radical Biology & Medicine·A M JentzschH K Biesalski
Nov 1, 1996·Free Radical Research·B Halliwell
Jan 1, 1997·Free Radical Biology & Medicine·H BussC C Winterbourn
Feb 12, 1998·Journal of Photochemistry and Photobiology. B, Biology·E E KelleyC P Burns
Jul 13, 2001·The Journal of Biological Chemistry·R V RaoD E Bredesen
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·M LevineJ Morrow
Sep 5, 2001·Nature Cell Biology·L RavagnanG Kroemer
Oct 5, 2001·Expert Opinion on Pharmacotherapy·C H SibataT J Kinsella
Mar 21, 2002·Journal of Clinical Laser Medicine & Surgery·Thomas J Dougherty
Nov 28, 2002·Breast Cancer Research and Treatment·M L LesperanceA Hoffer
Mar 8, 2003·Blood·Isabel Perez-CruzDavid W Golde
Nov 6, 2003·International Journal of Cancer. Journal International Du Cancer·Juergen FrankDebra K Kelleher
Dec 30, 2003·International Journal of Cancer. Journal International Du Cancer·Christine LambertJuergen Frank
Feb 21, 2004·Free Radical Biology & Medicine·Philip E MorganMichael J Davies
Feb 12, 2005·Antioxidants & Redox Signaling·Giuseppe FilomeniMaria R Ciriolo

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Citations

Dec 17, 2008·Photochemistry and Photobiology·Mohammad Reza Lornejad-SchäferJürgen Frank
Mar 20, 2012·Journal of Photochemistry and Photobiology. B, Biology·Su-juan ZhangXiao Liu
May 28, 2011·CA: a Cancer Journal for Clinicians·Patrizia AgostinisJakub Golab
Feb 4, 2014·Journal of Biochemical and Molecular Toxicology·Hang QiYoshimasa Nakamura
Jan 29, 2014·Cell Biology International·Rafael de Paula RodriguesNewton Soares da Silva
Aug 3, 2006·Nature Clinical Practice. Gastroenterology & Hepatology·Maria-Anna Ortner, Gian Dorta
Feb 21, 2013·Clinical Endoscopy·Il YoonYoung Key Shim

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