PMID: 589439Nov 4, 1977

Ascorbic acid transport by a clonal line of pheochromocytoma cells

Brain Research
R Spector, L A Greene

Abstract

A clonal line of rat pheochromocytoma cells was used as a model of noradrenergic tissue to study ascorbic acid transport. These cells were used because, like sympathetic neurons, they synthesize large amounts of noradrenaline in the presence of ascorbate, they respond to nerve growth factor with the production of neurites and they release, store and take up catecholamines. In these cells, both with and without nerve growth factor (NGF) treatment, [14C]ascorbic acid was concentrated by a stereospecific saturable, energy dependent transport system that could be described by a Michaelis-Menten transport model. The Kt and Vmax for ascorbic acid were approximately 0.03 mM and 0.3 nmole per min per mg protein respectively for both untreated and NGF-treated cells. The ability of the cells to concentrate ascorbic acid was not due to intracellular binding. Cells untreated with NGF and loaded with [14C]ascorbic acid to a concentration of 5.6 nmoles per mg protein retained only 6% of the initial intracellular [14C]ascorbic acid after the 24 h in normal growth medium. Thus, although pheochromocytoma cells contain an ascorbate concentrating system, optimal production of noradrenaline requires ascorbate in the medium.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Feb 14, 1975·Science·R Spector, A V Lorenzo
Jan 1, 1971·Clinica Chimica Acta; International Journal of Clinical Chemistry·D HornigO Wiss
Oct 15, 1965·Experientia·M GoldsteinS Sirlin
Jul 1, 1966·Experimental Eye Research·H Heath, R Fiddick
Feb 1, 1954·The Journal of Experimental Medicine·R DULBECCO, M VOGT

Citations

Apr 1, 1992·Journal of Bioenergetics and Biomembranes·A EscobarM T Núñez
Oct 1, 1990·Neurochemical Research·J X WilsonS J Dixon
Oct 1, 1984·Molecular and Cellular Endocrinology·C L Bethea, S L Kozak
Dec 1, 1986·Molecular and Cellular Endocrinology·E I CullenB A Eipper
Nov 1, 1987·Molecular and Cellular Endocrinology·C L BetheaS L Kozak
Oct 1, 1980·The Journal of Clinical Investigation·Reynold Spector
Aug 8, 2013·Endocrine-related Cancer·Alessio ImperialeIzzie-Jacques Namer
Jan 15, 2014·Molecular Brain·Reynold Spector, Conrad E Johanson
Mar 10, 2015·Experimental Neurology·Reynold SpectorConrad E Johanson
Dec 1, 1993·European Journal of Biochemistry·R RoskoskiL M Roskoski
Oct 1, 1988·Journal of Neurochemistry·H Wilgus, R Roskoski
Jul 11, 1983·FEBS Letters·M A Levine, H B Pollard
Nov 1, 1987·Journal of Andrology·W H Moger

Related Concepts

Adrenal Cancer
Magnorbin
Neurite Outgrowth Factors
Pheochromocytoma, Extra-Adrenal

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