ASE-1: a novel protein of the fibrillar centres of the nucleolus and nucleolus organizer region of mitotic chromosomes

Chromosoma
C WhiteheadJ B Rattner

Abstract

A novel nucleolar component has been identified and cloned using a human autoimmune serum. This antigen, as inferred from the cDNA sequence, is an Mr 55000 protein. Immuno blot analysis, however, of both the native protein and the in vitro translation products of the cDNA showed that they migrate on SDS-PAGE at an apparent molecular mass of 90000 A BLAST search using the cDNA sequence indicated that it is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC-1. An open reading frame, without a translational start site, had been observed by others in this region of the chromosome 19 (19q13.3) and the putative protein was termed ASE-1 (Anti-Sense to ERCC-1). Our cDNA is a full-length equivalent of that open reading frame. ASE-1 was found to contain two domains that are present in a number of nucleolar specific proteins originating from a variety of organisms: a glycine-, arginine- and phenylalanine-rich putative nucleotide interaction domain and an alternating basic/acidic region. Indirect immunofluorescence analysis using antibodies generated to cloned regions of ASE-1 indicated that this protein occurs at the fibrillar centres of the nucleolus in interphase, the putative sites of rDNA transcription,...Continue Reading

Citations

Jun 2, 2005·Trends in Immunology·Kathleen L McGuire, David S Holmes
Jul 1, 2006·Molecular and Cellular Biology·Kostya I PanovJoost C B M Zomerdijk
Oct 11, 2005·Neuro-oncology·Margaret WrenschJohn K Wiencke
Jan 6, 2006·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Tomotaka SugimuraKanji Ishizaki
Nov 30, 2006·International Journal of Cancer. Journal International Du Cancer·Annette VangstedUlla Vogel
Sep 3, 2011·European Journal of Haematology·Annette VangstedUlla Vogel
Oct 27, 2004·Seminars in Arthritis and Rheumatism·Yaniv ShererYehuda Shoenfeld
Feb 18, 2012·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Jiaoyang YinXinxin Li
Feb 24, 2001·Lupus·S EdworthyJ B Rattner
Jun 6, 2018·Laboratory Medicine·Lucia M SurGenel Sur
Jun 19, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hyo-Sung JeonJae Yong Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.