Abstract
Apomixis results in asexual seed formation where progeny are identical to the maternal plant. In ovules of apomictic species of the Hieracium subgenus Pilosella, meiosis of the megaspore mother cell generates four megaspores. Aposporous initial (AI) cells form during meiosis in most ovules. The sexual pathway terminates during functional megaspore (FM) differentiation, when an enlarged AI undergoes mitosis to form an aposporous female gametophyte. Then, the mitotically programmed FM dies along with the three other megaspores by unknown mechanisms. Transcriptomes of laser-dissected AIs, ovule cells, and ovaries from apomicts and AI-deficient mutants were analyzed to understand the pathways involved. The steps leading to AI mitosis and sexual pathway termination were determined using antibodies against arabinogalactan protein epitopes found to mark both sexual and aposporous female gametophyte lineages at inception. At most, four AIs differentiated near developing megaspores. The first expanding AI cell to contact the FM formed a functional AI that underwent mitosis soon after megaspore degeneration. Transcriptome analyses indicated that the enlarged, laser-captured AIs were arrested in the S/G2 phase of the cell cycle and were m...Continue Reading
Citations
Jan 25, 2019·Protoplasma·Tengfei MaJie Zhao
May 8, 2019·Journal of Experimental Botany·Jorge LoraMathew R Tucker
Jun 5, 2019·Plant Signaling & Behavior·Martina JuranićAnna M Koltunow
Jun 12, 2019·Plants·Andrea BassoGiulio Galla
Apr 20, 2019·Frontiers in Plant Science·Diego Hojsgaard, Elvira Hörandl
Nov 19, 2019·Frontiers in Plant Science·Laura G WilkinsonMatthew R Tucker
Mar 11, 2020·Frontiers in Plant Science·Fuad Bahrul UlumElvira Hörandl
Jun 19, 2020·Plant Methods·Martina JuranićAnna M G Koltunow
Sep 16, 2020·Genes·Sam W HendersonAnna M G Koltunow
Mar 25, 2019·Frontiers in Plant Science·Pankaj KaushalDevendra R Malaviya
Apr 16, 2020·Genes·Diego Hojsgaard
Jul 12, 2019·Journal of Experimental Botany·Luise ZühlAnja Schmidt
Apr 5, 2020·Genes·Anja Schmidt
Nov 19, 2020·BMC Plant Biology·Birthe H BarkeElvira Hörandl
Dec 23, 2020·International Journal of Molecular Sciences·Bartosz J PłachnoJanusz Kocki
Jan 25, 2020·Trends in Genetics : TIG·Peggy Ozias-Akins, Joann A Conner
Apr 5, 2021·Journal of Plant Research·Agnieszka Barbara JanasKrystyna Musiał
Aug 24, 2021·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Hui-Hui JiaWen-Wu Guo