ASF1 is required to load histones on the HIRA complex in preparation of paternal chromatin assembly at fertilization

Epigenetics & Chromatin
Béatrice HorardB Loppin

Abstract

Anti-Silencing Factor 1 (ASF1) is a conserved H3-H4 histone chaperone involved in both Replication-Coupled and Replication-Independent (RI) nucleosome assembly pathways. At DNA replication forks, ASF1 plays an important role in regulating the supply of H3.1/2 and H4 to the CAF-1 chromatin assembly complex. ASF1 also provides H3.3-H4 dimers to HIRA and DAXX chaperones for RI nucleosome assembly. The early Drosophila embryo is an attractive system to study chromatin assembly in a developmental context. The formation of a diploid zygote begins with the unique, genome-wide RI assembly of paternal chromatin following sperm protamine eviction. Then, within the same cytoplasm, syncytial embryonic nuclei undergo a series of rapid, synchronous S and M phases to form the blastoderm embryo. Here, we have investigated the implication of ASF1 in these two distinct assembly processes. We show that depletion of the maternal pool of ASF1 with a specific shRNA induces a fully penetrant, maternal effect embryo lethal phenotype. Unexpectedly, despite the depletion of ASF1 protein to undetectable levels, we show that asf1 knocked-down (KD) embryos can develop to various stages, thus demonstrating that ASF1 is not absolutely required for the amplif...Continue Reading

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Citations

Dec 14, 2018·Genes·Louis-Alexandre FournierPeter C Stirling
Sep 9, 2020·Nature Structural & Molecular Biology·Júlia TornéGeneviève Almouzni
Jul 21, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Nandini Karthik, Reshma Taneja
Sep 29, 2019·Scientific Reports·Yunsook MinYeonhee Choi
Aug 29, 2020·Annual Review of Genetics·Benjamin Loppin, Frédéric Berger
Mar 24, 2021·Molecular and Cellular Biology·Ann K Hogan, Daniel R Foltz

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Methods Mentioned

BETA
transgenic
co-immunoprecipitation
PCR
immunoprecipitation

Software Mentioned

Adobe Illustrator
Adobe Photoshop

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