Apr 9, 2010

Asna1/TRC40-mediated membrane insertion of tail-anchored proteins

Journal of Cell Science
Vincenzo FavaloroBernhard Dobberstein

Abstract

Tail-anchored (TA) proteins insert post-translationally into the membrane of the endoplasmic reticulum (ER) and span the membrane by their C-terminal transmembrane domain. We have reconstituted membrane insertion of TA proteins from recombinant Asna1/TA protein complexes and ER-derived membranes. Our data show that Asna1 can mediate membrane insertion of RAMP4 and Sec61beta without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the ER membrane. By contrast, membrane insertion of cytochrome b5 can proceed independently of Asna1 and nucleotides.

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Citations

Mentioned in this Paper

ASNA1 gene
Tissue Membrane
Carboxy-Terminal Amino Acid
Chimeric Proteins, Recombinant
Microsomes
Complex (molecular entity)
Transmembrane Domain
Endopeptidases
ASNA1 protein, human
Proteolytic Enzyme

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