Aspalathin, a dihydrochalcone C-glucoside, protects H9c2 cardiomyocytes against high glucose-induced shifts in substrate preference and apoptosis

Molecular Nutrition & Food Research
Rabia JohnsonJohan Louw

Abstract

Energy deprivation in the myocardium is associated with impaired heart function. This study aim to investigate if aspalathin can ameliorate hyperglycemic-induced shift in substrate preference and protect the myocardium against cell apoptosis. H9c2 cells were exposed to, either normal (5.5 mM) or high (33 mM) glucose concentrations for 48 hours. Thereafter, cells exposed to 33 mM glucose were treated with metformin (1 μM) or aspalathin (1 μM), as well as a combination of metformin and aspalathin for 6 hours. In vitro studies revealed that aspalathin improved glucose metabolism by decreasing fatty acid uptake and subsequent β-oxidation through the decreased expression of pAMPK (Thr(172) ) and CPT1, while increasing ACC and GLUT4 expression. Aspalathin inhibited high glucose-induced loss of membrane potential in H9c2 cells as observed by an increase in JC-1 ratio (orange\red fluorescence) and decreased apoptosis by reducing intracellular ROS and DNA nick formation, while increasing glutathione, superoxide dismutase, UCP2 and Bcl-2\Bax ratio. Our study provides evidence that aspalathin increases glucose oxidation and modulates fatty acid utilization producing a favorable substrate shift in H9c2 cardiomyocytes exposed to high glucos...Continue Reading

References

Apr 1, 1997·Cardiovascular Research·W C StanleyJ G McCormack
Oct 29, 2000·American Journal of Physiology. Endocrinology and Metabolism·D D BelkeD L Severson
Apr 30, 2003·Diabetes·Charles M AlexanderUNKNOWN National Cholesterol Education Program (NCEP)
Aug 16, 2005·Cell Metabolism·Martin D Brand, Telma C Esteves
Sep 28, 2005·Diabetes Care·Silvio E Inzucchi
Feb 7, 2006·Journal of the American College of Cardiology·Pilar HerreroRobert J Gropler
Mar 11, 2006·Diabetes Care·Sergio Muntoni
Sep 22, 2006·Molecular and Cellular Biochemistry·Susan L M CoortJoost J F P Luiken
May 8, 2009·Pediatrics International : Official Journal of the Japan Pediatric Society·Haruna BabaToshiaki Shimizu
May 26, 2009·American Journal of Epidemiology·Gemma Flores-MateoMaría-Isabel Covas
Dec 29, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mohammad Ali Esmaeili, Ali Sonboli
Jan 21, 2010·Physiological Reviews·Gary D LopaschukWilliam C Stanley
Apr 16, 2010·Canadian Journal of Physiology and Pharmacology·Madhu KhullarPawan K Singal
Apr 28, 2010·Expert Review of Neurotherapeutics·Altaf S DarveshCornelis J Van der Schyf
Jun 19, 2010·International Journal of Molecular Sciences·Omotayo Owomofoyon ErejuwaSunil Gurtu
Aug 19, 2010·Oxidative Medicine and Cellular Longevity·Kanti Bhooshan Pandey, Syed Ibrahim Rizvi
Jan 21, 2011·Current Pharmaceutical Design·Fan JiangGregory J Dusting
Jan 26, 2012·The Journal of Biological Chemistry·Suryakant K Niture, Anil K Jaiswal
Jul 4, 2012·Molecular and Cellular Endocrinology·Jeevan NagendranJason R B Dyck
Sep 11, 2012·Biochimica Et Biophysica Acta·Natasha Fillmore, Gary D Lopaschuk
Sep 27, 2012·The Journal of Pathology·David M Ansley, Baohua Wang
Oct 23, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·C J F MullerJ Louw
Dec 12, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Wei ChenMichael Wink
Dec 15, 2012·European Journal of Nutrition·Myoung Jin SonKazumi Yagasaki
Feb 2, 2013·Diabetes Research and Clinical Practice·T-I LeeY-J Chen
May 4, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·S E MazibukoJ Louw
Jul 19, 2013·Expert Opinion on Drug Delivery·Shengpeng WangYitao Wang
Aug 14, 2013·Journal of Diabetes and Metabolic Disorders·Zahra BahadoranFereidoun Azizi
Nov 26, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·P V DludlaR Johnson
Nov 26, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiao SunXiao-bo Sun
Nov 28, 2013·Journal of the American Heart Association·Marina BayevaHossein Ardehali
Dec 21, 2013·Journal of Natural Products·Ze HanJan H van der Westhuizen
Oct 1, 2014·PloS One·Gemma Flores-MateoUNKNOWN PREDIMED Study Investigators

❮ Previous
Next ❯

Citations

Feb 2, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rabia JohnsonJohan Louw
May 23, 2017·Nutrients·Rosanna MatteraRoberto Bei
Jan 19, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Phiwayinkosi V DludlaRabia Johnson
Sep 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rabia JohnsonChristo John Frederick Muller
Apr 7, 2018·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Phiwayinkosi V DludlaBongani B Nkambule
Dec 5, 2018·Journal of Biochemical and Molecular Toxicology·Ravichandra Shivalingappa DavargaonSubramanyam Muthangi V V
May 26, 2020·Journal of Cellular and Molecular Medicine·Dan YanZhengyuan Xia
May 28, 2020·Journal of Cellular Physiology·Danielle A MillarChristo J F Muller
Apr 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xi ZhangHong Zheng
Dec 28, 2019·International Journal of Molecular Sciences·Gideon Gatluak KangAbishek Bommannan Santhakumar
May 21, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Junqin ChenAnath Shalev
Jul 8, 2017·Endocrinology and Metabolism·Lamario J WilliamsAdam R Wende
Nov 30, 2018·Molecular Medicine Reports·Qian WangYan Meng
Jan 19, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Victoria L NasciAlison J Kriegel
Dec 7, 2016·Electrophoresis·William J ArriesAndré de Villiers
Apr 8, 2018·In Vitro Cellular & Developmental Biology. Animal·Yi ShenLiang-Yi Si
Sep 1, 2018·Heart Failure Reviews·Nonhlakanipho F SangweniRabia Johnson
Feb 6, 2019·International Journal of Molecular Sciences·Sithandiwe E Mazibuko-MbejeChristo J F Muller
Apr 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Khanyisani ZiqubuSithandiwe E Mazibuko-Mbeje
Oct 13, 2020·Frontiers in Physiology·Mario SchubertKaomei Guan
Dec 22, 2020·Journal of Cellular Physiology·Liang LiXi-Long Zheng
Apr 3, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Nonhlakanipho F SangweniRabia Johnson
Jul 25, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sithandiwe E Mazibuko-MbejeChristo J F Muller
Aug 5, 2021·European Journal of Pharmacology·Wen DingQi-Zhu Tang
Nov 9, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C R AnandSrinivas Gopala

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis