Asparaginase conjugated magnetic nanoparticles used for reducing acrylamide formation in food model system

Bioresource Technology
Shahenvaz AlamSunil Kumar Khare

Abstract

Acrylamide is a potent carcinogen and neurotoxin formed by the Maillard reaction when l-asparagine reacts with starch at high temperature. It is formed in food materials mainly deep fried and bakery products. Enzymatic pretreatment of these food products with asparaginase enzyme leads to reduction in acrylamide. However, enzymatic process is quite expensive due to high cost, low catalytic efficiency as well as problem with enzyme reusability. Present work deals with these problems by exploring l-asparaginase from Bacillus aryabhattai. Asparaginase enzyme was immobilized on APTES modified magnetic nanoparticles. It was found to be more than three-fold increase their thermal stability from free enzyme and retained 90% activity after fifth cycle. The immobilized enzyme also showed better affinity towards its substrate. During pretreatment of asparagine in a starch-asparagine food model system and it was clearly demonstrated that asparaginase nanoconjugates had reduced the formation of acrylamide by more than 90% within 30 min.

Citations

Mar 26, 2019·Critical Reviews in Food Science and Nutrition·Agnieszka KoszuckaIlona Motyl
Jul 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nemi MalhotraChung-Der Hsiao
Feb 14, 2020·Archives of Microbiology·Faizan MuneerHabibullah Nadeem
Nov 4, 2020·Advances in Colloid and Interface Science·Zahra Mohammadi, Seid Mahdi Jafari
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·João C F NunesAna P M Tavares
Mar 23, 2021·Medicine in Drug Discovery·Nikky GoelSunil Kumar Khare
Sep 23, 2020·International Journal of Biological Macromolecules·Syeda Warisul FatimaSunil K Khare
May 1, 2021·International Journal of Environmental Research and Public Health·Cristina SarionAdriana Dabija
Jul 3, 2021·Frontiers in Genetics·Md ZeyaullahRazi Ahmad
Nov 28, 2021·Pharmaceutics·Francisca Villanueva-FloresAlejandro Huerta-Saquero

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