PMID: 25738356Mar 5, 2015Paper

Asparaginase induces apoptosis and cytoprotective autophagy in chronic myeloid leukemia cells

Oncotarget
Ping SongDianwen Ju

Abstract

The antitumor enzyme asparaginase, which targets essential amino acid L-asparagine and catalyzes it to L-aspartic acid and ammonia, has been used for years in the treatment of acute lymphoblastic leukemia (ALL), subtypes of myeloid leukemia and T-cell lymphomas, whereas the anti-chronic myeloid leukemia (CML) effect of asparaginase and its underlying mechanism has not been completely elucidated. We have shown here that asparaginase induced significant growth inhibition and apoptosis in K562 and KU812 cells. Apart from induction of apoptosis, we reported for the first time that asparaginase induced autophagic response in K562 and KU812 cells as evidenced by the formation of autophagosome, microtubule-associated protein light chain 3 (LC3)-positive autophagy-like vacuoles, and the upregulation of LC3-II. Further study suggested that the Akt/mTOR (mammalian target of rapamycin) and Erk (extracellular signal-regulated kinase) signaling pathway were involved in asparaginase-induced autophagy in K562 cells. Moreover, blocking autophagy using pharmacological inhibitors LY294002, chloroquine (CQ) and quinacrine (QN) enhanced asparaginase-induced cell death and apoptosis, indicating the cytoprotective role of autophagy in asparaginase-t...Continue Reading

References

Jan 20, 2007·The Journal of Clinical Investigation·Ravi K AmaravadiCraig B Thompson
Mar 17, 2007·Critical Reviews in Biotechnology·Neelam VermaSneh Anand
Mar 24, 2007·The Journal of Clinical Investigation·Shotaro IwamotoDario Campana
Aug 25, 2007·Nature Reviews. Molecular Cell Biology·M Chiara MaiuriGuido Kroemer
Oct 15, 2008·Molecular Cancer Therapeutics·Philip L LorenziJohn N Weinstein
Mar 28, 2009·Cell Death and Differentiation·A Eisenberg-LernerA Kimchi
Jan 20, 2010·FEBS Letters·Chang Hwa JungDo-Hyung Kim
May 28, 2011·Science·Carmine SettembreAndrea Ballabio
Aug 23, 2011·Recent Patents on Anti-cancer Drug Discovery·Daniele CoviniClaudia Scotti
Nov 10, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David MarinLetizia Foroni
Dec 15, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Saurabh BansalBishwajit Kundu
Feb 16, 2012·Journal of Cellular and Molecular Medicine·Minshu YuElise C Kohn
Sep 6, 2012·Expert Opinion on Biological Therapy·Wanda SalzerMalcolm Smith
Sep 6, 2012·Autophagy·David ColecchiaMario Chiariello
Oct 24, 2012·Cancer Management and Research·Jennifer S CarewSteffan T Nawrocki
Dec 14, 2012·World Journal of Gastroenterology : WJG·Györgyi Műzes, Ferenc Sipos
Dec 18, 2012·Breast Cancer Research and Treatment·Josefine BostnerOlle Stål
Jul 3, 2013·Seminars in Cancer Biology·Séverine LorinPatrice Codogno
Aug 15, 2013·Current Cancer Drug Targets·Gudmundur V HelgasonTessa L Holyoake
Oct 4, 2013·Nature·Olatz PampliegaAna Maria Cuervo
Nov 19, 2013·Nature Communications·Nuria Martinez-LopezRajat Singh
Dec 11, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Philippe RousselotFrançois-Xavier Mahon
Dec 18, 2013·Cell Research·Ryan C RussellKun-Liang Guan
Jan 18, 2014·Chemico-biological Interactions·Bibhas RoyTapas K Maiti
Feb 8, 2014·Molecular Cancer Research : MCR·Eduard H PanosyanJoseph L Lasky
Feb 12, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Dan DouerAnn M Mohrbacher
Apr 15, 2014·American Journal of Hematology·Elias Jabbour, Hagop Kantarjian
May 23, 2014·Drug Discovery Today. Technologies·Stefan BalabanovTim H Brümmendorf

❮ Previous
Next ❯

Citations

Oct 12, 2018·Medicinal Research Reviews·Hendrik FolkertsValerie R Wiersma
Mar 30, 2017·Applied Microbiology and Biotechnology·Yunxiang JiDianwen Ju
Feb 23, 2017·International Journal of Cancer. Journal International Du Cancer·Ju Han SongTae Sung Kim
Jan 14, 2017·International Journal of Molecular Sciences·Jian-Guang YangGang Zhou
Mar 15, 2018·Cancer Metastasis Reviews·Chandan Kanta DasDonat Kögel
Aug 8, 2018·International Journal of Molecular Sciences·Xian Zeng, Dianwen Ju
Dec 9, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yubin LiDianwen Ju
Jun 3, 2016·Applied Microbiology and Biotechnology·Boyang ZhangDianwen Ju
May 26, 2017·PloS One·Stefania BelvisoMiranda Menniti
Mar 31, 2017·Cell Death & Disease·Ping SongDianwen Ju
Oct 11, 2017·Current Medicinal Chemistry·Vadim S PokrovskyElena V Lukasheva
Nov 3, 2017·Acta Crystallographica. Section D, Structural Biology·Jingxu GuoMuhummad Akhtar
Dec 29, 2020·International Journal of Biological Macromolecules·Sahand Mazloum-RavasanFarshad Darvishi
Feb 11, 2020·Applied Microbiology and Biotechnology·Rakhi DhankharPooja Gulati
Jan 1, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Verica PaunovicLjubica Harhaji-Trajkovic
Oct 30, 2020·International Journal of Molecular Sciences·Polina V ShnaiderVictoria O Shender
Jan 30, 2021·Frontiers in Pharmacology·Ziyu WangDianwen Ju
Aug 25, 2017·Biochemical and Biophysical Research Communications·Dmitry D ZhdanovNikolai N Sokolov
May 4, 2021·Frontiers in Pharmacology·Marco CordaniSuresh Kumar
Jun 17, 2020·ACS Applied Materials & Interfaces·Jing GuQunyou Tan
Sep 18, 2021·Molecular Carcinogenesis·Carly S WilderJohn DiGiovanni

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.