In membrane proteins, the extent to which polarity, hydrogen bonding, and van der Waals packing interactions of the buried, internal residues direct protein folding and association of transmembrane segments is poorly understood. The energetics associated with these various interactions should differ substantially between membrane versus water-soluble proteins. To help evaluate these energetics, we have altered a water-soluble, two-stranded coiled-coil peptide to render its sequence soluble in membranes. The membrane-soluble peptide associates in a monomer-dimer-trimer equilibrium, in which the trimer predominates at the highest peptide/detergent ratios. The oligomers are stabilized by a buried Asn side chain. Mutation of this Asn to Val essentially eliminates oligomerization of the membrane-soluble peptide. Thus, within a membrane-like environment, interactions involving a polar Asn side chain provide a strong thermodynamic driving force for membrane helix association.
Replica exchange Monte-Carlo simulations of helix bundle membrane proteins: rotational parameters of helices
Assembly of light-harvesting bacteriochlorophyll in a model transmembrane helix in its natural environment
Modulation of cell function by small transmembrane proteins modeled on the bovine papillomavirus E5 protein
A supramolecular assembly of side-by-side polyimidazole tripod coils stabilized by pi-pi stacking and unique boric acid templated hydrogen bonding interactions
Lipid-mediated interactions tune the association of glycophorin A helix and its disruptive mutants in membranes
Sequence dependent lipid-mediated effects modulate the dimerization of ErbB2 and its associative mutants
Transmembrane polar interactions are required for signaling in the Escherichia coli sensor kinase PhoQ
Oligomerization is crucial for the stability and function of heme oxygenase-1 in the endoplasmic reticulum
β-Subunit of the Ostα-Ostβ organic solute transporter is required not only for heterodimerization and trafficking but also for function.
Dimer interface of transmembrane domains for neu/erbB-2 receptor dimerization and transforming activation: a model revealed by molecular dynamics simulations
Transmembrane peptides from tyrosine kinase receptor. Mutation-related behavior in a lipid bilayer investigated by molecular dynamics simulations
Sequence-specific retention and regulated integration of a nascent membrane protein by the endoplasmic reticulum Sec61 translocon
Role of intramembrane charged residues in the quality control of unassembled T-cell receptor alpha-chains at the endoplasmic reticulum
Use of thiol-disulfide equilibria to measure the energetics of assembly of transmembrane helices in phospholipid bilayers
The Calpha ---H...O hydrogen bond: a determinant of stability and specificity in transmembrane helix interactions
Packing contacts can mediate highly specific interactions between artificial transmembrane proteins and the PDGFbeta receptor
Transmembrane pores formed by synthetic p-octiphenyl beta-barrels with internal carboxylate clusters: regulation of ion transport by pH and Mg(2+)- complexed 8-aminonaphthalene-1,3,6-trisulfonate
Potential of mean force analysis of the self-association of leucine-rich transmembrane α-helices: difference between atomistic and coarse-grained simulations
Structural basis for tetraspanin functions as revealed by the cryo-EM structure of uroplakin complexes at 6-A resolution
Asn- and Asp-mediated interactions between transmembrane helices during translocon-mediated membrane protein assembly
Alternate interfaces may mediate homomeric and heteromeric assembly in the transmembrane domains of SNARE proteins
Forster resonance energy transfer in liposomes: measurements of transmembrane helix dimerization in the native bilayer environment
Screening for transmembrane association in divisome proteins using TOXGREEN, a high-throughput variant of the TOXCAT assay
Experimental and computational evaluation of forces directing the association of transmembrane helices
Essential role for the putative S6 inner pore region in the activation gating of the human TRPA1 channel
Asn183 in alpha5 is essential for oligomerisation and toxicity of the Bacillus thuringiensis Cry4Ba toxin
The interplay of functional tuning, drug resistance, and thermodynamic stability in the evolution of the M2 proton channel from the influenza A virus
Specific heteromeric association of four transmembrane peptides derived from platelet glycoprotein Ib-IX complex
Synergistic interactions between aqueous and membrane domains of a designed protein determine its fold and stability
Pentameric ligand-gated ion channels exhibit distinct transmembrane domain archetypes for folding/expression and function
Identifying ionic interactions within a membrane using BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions
Interhelical hydrogen bonds and spatial motifs in membrane proteins: polar clamps and serine zippers
Determination of membrane protein stability via thermodynamic coupling of folding to thiol-disulfide interchange
Missense mutations in transmembrane domains of proteins: phenotypic propensity of polar residues for human disease
An activating mutation in the transmembrane domain of the granulocyte colony-stimulating factor receptor in patients with acute myeloid leukemia
Hydrogen bonding in a model bacteriochlorophyll-binding site drives assembly of light harvesting complex.
Mutations within an intramembrane leucine heptad repeat disrupt oligomer formation of the rat GABA transporter 1.
A FlAsH-tetracysteine assay for quantifying the association and orientation of transmembrane α-helices
Rendezvous in a membrane: close packing, hydrogen bonding, and the formation of transmembrane helix oligomers
The dimeric transmembrane domain of prolyl dipeptidase DPP-IV contributes to its quaternary structure and enzymatic activities
Influence of hydrophobic matching on association of model transmembrane fragments containing a minimised glycophorin A dimerisation motif
Fluorophores, environments, and quantification techniques in the analysis of transmembrane helix interaction using FRET
Toll-like receptor 3 transmembrane domain is able to perform various homotypic interactions: an NMR structural study
Efficient access to enantiopure γ4-amino acids with proteinogenic side-chains and structural investigation of γ4-Asn and γ4-Ser in hybrid peptide helices
Single methyl groups can act as toggle switches to specify transmembrane Protein-protein interactions
Aph-1 contributes to the stabilization and trafficking of the gamma-secretase complex through mechanisms involving intermolecular and intramolecular interactions
Peptides as transmembrane segments: decrypting the determinants for helix-helix interactions in membrane proteins
Transmembrane helices that form two opposite homodimeric interactions: an asparagine scan study of alphaM and beta2 integrins
A serine in the first transmembrane domain of the human E3 ubiquitin ligase MARCH9 is critical for down-regulation of its protein substrates
The transmembrane domain of glycoprotein Ibbeta is critical to efficient expression of glycoprotein Ib-IX complex in the plasma membrane
P2X5 subunit assembly requires scaffolding by the second transmembrane domain and a conserved aspartate
Design of self-assembling transmembrane helical bundles to elucidate principles required for membrane protein folding and ion transport
His499 Regulates Dimerization and Prevents Oncogenic Activation by Asparagine Mutations of the Human Thrombopoietin Receptor.
Interhelical packing in detergent micelles. Folding of a cystic fibrosis transmembrane conductance regulator construct
Insertion and organization within membranes of the delta-endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant helix 4 peptide
Structural and functional features of the transmembrane domain of the Na,K-ATPase beta subunit revealed by tryptophan scanning
In vitro selection of membrane-spanning leucine zipper protein-protein interaction motifs using POSSYCCAT
Identification and characterization of an amphipathic leucine zipper-like motif in Escherichia coli toxin hemolysin E. Plausible role in the assembly and membrane destabilization
Sequence-specific dimerization of the transmembrane domain of the "BH3-only" protein BNIP3 in membranes and detergent
The interface between self-assembling erythropoietin receptor transmembrane segments corresponds to a membrane-spanning leucine zipper.
The affinity of GXXXG motifs in transmembrane helix-helix interactions is modulated by long-range communication.
Characterization of the proto-oncogenic and mutant forms of the transmembrane region of Neu in micelles.
A knowledge-based potential highlights unique features of membrane α-helical and β-barrel protein insertion and folding
The identification of a minimal dimerization motif QXXS that enables homo- and hetero-association of transmembrane helices in vivo
Cysteine scanning mutagenesis and disulfide mapping studies of the TatA component of the bacterial twin arginine translocase
Novel free energy calculations to explore mechanisms and energetics of membrane protein structure and function
The typically mitochondrial DNA-encoded ATP6 subunit of the F1F0-ATPase is encoded by a nuclear gene in Chlamydomonas reinhardtii
Control of conformational equilibria in the human B2 bradykinin receptor. Modeling of nonpeptidic ligand action and comparison to the rhodopsin structure
Directly Activating the Integrin αIIbβ3 Initiates Outside-In Signaling by Causing αIIbβ3 Clustering.
Small-residue packing motifs modulate the structure and function of a minimal de novo membrane protein
Self-association of transmembrane alpha-helices in model membranes: importance of helix orientation and role of hydrophobic mismatch
Structure and drug binding of the SARS-CoV-2 envelope protein transmembrane domain in lipid bilayers.
In silico screening of mutational effects on transmembrane helix dimerization: insights from rigid-body docking and molecular dynamics simulations
Molecular recognition at the membrane-water interface: controlling integral peptide helices by off-membrane nucleobase pairing
A novel scoring function for predicting the conformations of tightly packed pairs of transmembrane alpha-helices
Oligomerization of a peptide derived from the transmembrane region of the sodium pump gamma subunit: effect of the pathological mutation G41R
Structural aspects of oligomerization taking place between the transmembrane alpha-helices of bitopic membrane proteins
Peptide inhibitors use two related mechanisms to alter the apparent calcium affinity of the sarcoplasmic reticulum calcium pump
Peptide and protein building blocks for synthetic biology: from programming biomolecules to self-organized biomolecular systems
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
STING Receptor Agonists
Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.
Chronic Fatigue Syndrome
Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.
Spatio-Temporal Regulation of DNA Repair
DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.
Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.
Hereditary Sensory Autonomic Neuropathy
Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.
Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.
Neural Activity: Imaging
Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.
Applications of Molecular Barcoding
The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.