Aspartame-induced apoptosis in PC12 cells

Environmental Toxicology and Pharmacology
Yukari HorioMasaaki Kurasaki

Abstract

Aspartame is an artificial sweetner added to many low-calorie foods. The safety of aspartame remains controversial even though there are many studies on its risks. In this study, to understand the physiological effects of trace amounts of artificial sweetners on cells, the effects of aspartame on apoptosis were investigated using a PC12 cell system. In addition, the mechanism of apoptosis induced by aspartame in PC12 cells and effects on apoptotic factors such as cytochrome c, apoptosis-inducing factor, and caspase family proteins were studied by Western blotting and RT-PCR. Aspartame-induced apoptosis in PC12 cells in a dose-dependent manner. In addition, aspartame exposure increased the expressions of caspases 8 and 9, and cytochrome c. These results indicate that aspartame induces apoptosis mainly via mitochondrial pathway involved in apoptosis due to oxigen toxicity.

References

Mar 1, 1981·Toxicology Letters·H Ishii
Aug 1, 1993·The Journal of Cell Biology·A Batistatou, L A Greene
Oct 1, 1996·The Journal of Experimental Medicine·S A SusinG Kroemer
Nov 1, 1996·Journal of Neuropathology and Experimental Neurology·J W OlneyL N Robins
Jul 16, 1997·Journal of the National Cancer Institute·J G GurneyS Preston-Martin
Apr 30, 1998·Current Opinion in Cell Biology·D C Heimbrook, A Oliff
Aug 28, 1998·Science·D R Green, J C Reed
Sep 12, 1998·Science·J M Adams, S Cory
Jun 3, 1999·Brain Research. Molecular Brain Research·A WoodgateM Dragunow
Aug 13, 1999·Genes & Development·A GrossS J Korsmeyer
Jan 29, 2000·FEBS Letters·Y Tsujimoto, S Shimizu
Jun 2, 2000·Biochemical and Biophysical Research Communications·O YamanoshitaT Iwakuma
Oct 26, 2000·Nature·M O Hengartner
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·I SchmitzP H Krammer
Feb 13, 2001·Trends in Biochemical Sciences·J M Adams, S Cory
Nov 16, 2001·Biochemical Society Transactions·M Schuler, D R Green
Jan 24, 2004·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·J EmeritF Bricaire
Feb 28, 2004·Life Sciences·Miho AokiToshio Iwakuma
Mar 20, 2004·Nature Reviews. Drug Discovery·Kevin J BarnhamAshley I Bush
Nov 23, 2006·Annals of the New York Academy of Sciences·Fiorella BelpoggiFranco Minardi
Sep 7, 2007·Environmental Health Perspectives·Morando SoffrittiMichelina Lauriola
Mar 4, 2008·Trends in Cell Biology·Jerry E Chipuk, Douglas R Green
Apr 3, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Masaki KawakamiMasaaki Kurasaki
May 15, 2008·Biochimica Et Biophysica Acta·Wen-Chih ChengJ Marie Hardwick
Sep 15, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Renata AllevaMarco Tomasetti
Nov 9, 2010·Journal of Cardiac Failure·Roberta L DeBiasiPenny Clarke
Aug 9, 2011·Neurotoxicity Research·Omar M E Abdel-SalamJihan Seid Hussein
Feb 22, 2012·Basic & Clinical Pharmacology & Toxicology·Yongkun SunMasaaki Kurasaki
May 18, 2012·Cell Health and Cytoskeleton·Sathish Kumar Natarajan, Donald F Becker
Oct 26, 2012·The American Journal of Clinical Nutrition·Eva S SchernhammerDiane Feskanich

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Citations

Aug 8, 2015·Journal of Basic and Clinical Physiology and Pharmacology·Oluwatosin A Adaramoye, Olubukola O Akanni

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