Aspartate aminotransferase in synaptic and nonsynaptic mitochondria: differential effect of compounds that influence transient hetero-enzyme complex (metabolon) formation

Neurochemistry International
Mary C McKennaPenelope Bamford

Abstract

The enzyme aspartate aminotransferase (AAT) has a number of key roles in astrocytes and neurons in brain. An understanding of the regulation of AAT is important since AAT is involved in many aspects of glutamate metabolism including the synthesis of neurotransmitter glutamate. Mitochondrial AAT binds to a protein and lipids on the inner mitochondrial membrane and also forms a number of transient hetero-enzyme complexes with other enzymes. These complexes serve to facilitate metabolism by essentially channeling substrates and cofactors to other enzymes within the complex. The association and dissociation of transiently formed hetero-enzyme complexes may modulate enzyme activity in "real time" since these complexes are dynamically influenced by changes in the concentration of a number of key metabolites. The influence of several effectors that modulate AAT activity, either directly, or by altering the binding of AAT to mitochondrial lipids, or the association/dissociation into transient hetero-enzyme complexes was determined. The addition of palmitate, malate, citrate, glutamate, bovine serum albumin and Mg(2+) modulated AAT activity differently in synaptic and nonsynaptic mitochondria from brain. These findings suggest that AAT ...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·J C Lai, J B Clark
Dec 1, 1990·Neurochemical Research·M C McKennaF J Caprio
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P D BerkS Thung
Aug 1, 1990·Journal of Cellular Biochemistry·S BeeckmansL Kanarek
May 1, 1988·Journal of Neurochemistry·A J Cheeseman, J B Clark
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Feb 1, 1984·Annals of Neurology·A PlaitakisM D Yahr
Apr 1, 1984·Archives of Biochemistry and Biophysics·L A FahienM Marshall
Feb 1, 1983·Archives of Biochemistry and Biophysics·L A Fahien, E Kmiotek
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·N WestergaardA Schousboe
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·L M IsolaP D Berk
Jan 1, 1993·Developmental Neuroscience·A SchousboeL Hertz
Mar 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·S L ZhouP D Berk
Dec 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I SchousboeA Schousboe
Dec 1, 1995·Neurochemical Research·M C McKennaK G Kingwell
Sep 23, 1997·Glia·U SonnewaldA Schousboe
Mar 14, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·U SonnewaldA Schousboe
Apr 29, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·J MoranS Hurtado
Dec 18, 2001·Journal of Neuroscience Research·M YudkoffI Nissim
Apr 20, 2004·The European Journal of Neuroscience·Andrea Caballero-BenítezJulio Morán
May 13, 2005·Neurochemistry International·Marc YudkoffItzhak Nissim

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Citations

Oct 15, 2009·Cellular and Molecular Life Sciences : CMLS·Qian HanJianyong Li
Jan 30, 2010·BMC Biochemistry·Sergio GoncalvesPierre Rustin
May 26, 2009·Experimental Neurology·Janet M Dubinsky
Jul 30, 2015·Glia·Jakob D NissenHelle S Waagepetersen
Apr 5, 2014·Free Radical Research·Shinji OikawaTetsumori Yamashima
Jun 3, 2014·BioMed Research International·Alexander PanovVyacheslav Lyakhovich
Sep 12, 2007·Journal of Neuroscience Research·Mary C McKenna
Feb 15, 2017·Reviews in the Neurosciences·Sergei V FedorovichLudmila V Puchkova

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