Aspartate attenuates intestinal injury and inhibits TLR4 and NODs/NF-κB and p38 signaling in weaned pigs after LPS challenge

European Journal of Nutrition
Haibo WangShuang Li

Abstract

This study was conducted to investigate whether aspartate (Asp) could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury by modulating intestine inflammatory response. Twenty-four weaned piglets were divided into four treatments: (1) non-challenged control; (2) LPS-challenged control; (3) LPS + 0.5 % Asp; and (4) LPS + 1.0 % Asp. After feeding with control, 0.5 or 1.0 % Asp-supplemented diets for 21 days, pigs were injected intraperitoneally with saline or LPS. At 4 h postinjection, blood and intestine samples were obtained. Asp supplementation to LPS-challenged pigs improved intestinal morphology, indicated by higher jejunal and ileal villus height/crypt depth ratio and lower ileal crypt depth linearly or quadratically. Asp also improved intestinal barrier function, indicated by increased jejunal and ileal diamine oxidase activities as well as enhanced protein expression of jejunal claudin-1 linearly or quadratically. In addition, Asp decreased plasma, jejunal and ileal tumor necrosis factor-α concentration and ileal caspase-3 protein expression linearly and quadratically. Moreover, Asp down-regulated the mRNA expression of toll-like receptor 4 (TLR4) and nucleotide-binding oligomerization domain pro...Continue Reading

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Citations

Aug 24, 2017·Nutrients·Yulan LiuChien-An Andy Hu
Apr 24, 2018·BioMed Research International·Fang HeYuanyi Peng
Jan 30, 2021·Inflammatory Bowel Diseases·Maria Laura SantoruLuigi Atzori
Oct 24, 2020·Biomolecules & Therapeutics·Zhenling ZhangZhijun Duan
Jun 12, 2021·Frontiers in Veterinary Science·Tristan Chalvon-DemersayEtienne Corrent

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