PMID: 8955904Jan 1, 1996Paper

Aspartate identity of transfer RNAs

Biochimie
R GiegéD Moras

Abstract

Structure/function relationships accounting for specific tRNA charging by class II aspartyl-tRNA synthetases from Saccharomyces cerevisiae, Escherichia coli and Thermus thermophilus are reviewed. Effects directly linked to tRNA features are emphasized and aspects about synthetase contribution in expression of tRNA(Asp) identity are also covered. Major identity nucleotides conferring aspartate specificity to yeast, E coli and T thermophilus tRNAs comprise G34, U35, C36, C38 and G73, a set of nucleotides conserved in tRNA(Asp) molecules of other biological origin. Aspartate specificity can be enhanced by negative discrimination preventing, eg mischarging of native yeast tRNA(Asp by yeast arginyl-tRNA synthetase. In the yeast system crystallography shows that identity nucleotides are in contact with identity amino acids located in the catalytic and anticodon binding domains of the synthetase. Specificity of RNA/protein interaction involves a conformational change of the tRNA that optimizes the H-bonding potential of the identity signals on both partners of the complex. Mutation of identity nucleotides leads to decreased aspartylation efficiencies accompanied by a loss of specific H-bonds and an altered adaptation of tRNA on the sy...Continue Reading

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Citations

Jul 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hervé RoyDaniel Kern
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·D SöllM Ibba
May 16, 2009·The Journal of Biological Chemistry·Tania BourMagali Frugier
Mar 2, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Aron Szenes, Gábor Pál
Sep 14, 2011·Annual Review of Genetics·Tsutomu SuzukiTakeo Suzuki
Aug 31, 2000·Annual Review of Biochemistry·M Ibba, D Soll
Mar 21, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Martin Kollmar, Stefanie Mühlhausen
Apr 7, 2006·The Journal of Biological Chemistry·Aurélie FenderMarie Sissler
May 10, 2000·Journal of Molecular Biology·G Tocchini-ValentiniJ Abelson

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