Aspartic peptide hydrolases in Salmonella enterica serovar typhimurium

Journal of Bacteriology
R A LarsenC G Miller

Abstract

Two well-characterized enzymes in Salmonella enterica serovar Typhimurium and Escherichia coli are able to hydrolyze N-terminal aspartyl (Asp) dipeptides: peptidase B, a broad-specificity aminopeptidase, and peptidase E, an Asp-specific dipeptidase. A serovar Typhimurium strain lacking both of these enzymes, however, can still utilize most N-terminal Asp dipeptides as sources of amino acids, and extracts of such a strain contain additional enzymatic activities able to hydrolyze Asp dipeptides. Here we report two such activities from extracts of pepB pepE mutant strains of serovar Typhimurium identified by their ability to hydrolyze Asp-Leu. Although each of these activities hydrolyzes Asp-Leu at a measurable rate, the preferred substrates for both are N-terminal isoAsp peptides. One of the activities is a previously characterized isoAsp dipeptidase from E. coli, the product of the iadA gene. The other is the product of the serovar Typhimurium homolog of E. coli ybiK, a gene of previously unknown function. This gene product is a member of the N-terminal nucleophile structural family of amidohydrolases. Like most other members of this family, the mature enzyme is generated from a precursor protein by proteolytic cleavage and the ...Continue Reading

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Citations

Aug 5, 2003·Journal of Bacteriology·Daniel H Broder, Charles G Miller
Jan 2, 2007·Biochemical and Biophysical Research Communications·Anujith Kumar, Dipankar Nandi
May 27, 2006·Journal of Molecular Biology·Karolina MichalskaMariusz Jaskolski
Sep 20, 2007·FEMS Immunology and Medical Microbiology·Veerupaxagouda PatilDipankar Nandi
Apr 6, 2018·Journal of Molecular Modeling·Ronaldo Correia da SilvaEvonnildo Costa Gonçalves
Nov 11, 2010·Angewandte Chemie·Jason D FiedlerM G Finn
Jun 11, 2005·The Journal of Biological Chemistry·Karolina MichalskaMariusz Jaskolski
Apr 26, 2014·The Journal of Biological Chemistry·Alexander N PatanananSteven G Clarke
Jun 1, 2006·Microbiology·Janet C Waterhouse, Roy R B Russell
Mar 29, 2014·Biochemistry·Amanda M Schalk, Arnon Lavie

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