PMID: 590271Nov 15, 1977

Aspects of microenvironmental compartmentation. An evaluation of the influence of restricted diffusion, exclusion effects, and enzyme proximity on the overall efficiency of the sequential two-enzyme system malate dehydrogenase--citrate synthase in its soluble and immobilized form

European Journal of Biochemistry
A C Koch-SchmidtK Mosbach

Abstract

Soluble bifunctional enzyme aggregates have been prepared by cross-linking the sequential enzymes malate dehydrogenase (EC 1.1.1.37) and citrate synthase (EC 4.1.3.7) using glutaraldehyde. The kinetic behaviour of this two-enzyme system in its aggregated and non-aggregated form was studied both in free solution and immobilized on Sepharose beads. This study was undertaken in order to distinguish between the following two factors which may account for the increased efficiency found in general in co-immobilized consecutive two-enzyme systems: (a) closer proximity between the participating enzymes and (b) establishment of a favourable microenvironment (such as higher local intermediate concentration caused by increased diffusional hindrance in the gel phase). It was found that in spite of a reduction of the distance between the two enzymes in the aggregated form by an estimated factor of 10(3), no kinetic advantage (shorter lag phase or higher steady-state rate) could be detected compared to the corresponding system with the two enzymes not linked to each other. However, both systems immobilized to Sepharose reached the steady-state rate of citrate formation almost immediately, in contrast to the corresponding free systems which e...Continue Reading

References

Jan 1, 1976·Methods in Enzymology·K Mosbach, B Mattiasson
Jun 15, 1976·European Journal of Biochemistry·J Mowbray, V Moses
Jan 1, 1976·Methods in Enzymology·B Mattiasson, K Mosbach
Jan 1, 1973·Archives of Biochemistry and Biophysics·N B Oza, J D Shore
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·P A SREREK Mosbach
Jan 1, 1970·Acta Chemica Scandinavica·K Mosbach, B Mattiasson
Apr 14, 1971·Biochimica Et Biophysica Acta·B Mattiasson, K Mosbach
Apr 2, 1973·European Journal of Biochemistry·R MacnabJ Mowbray
Jan 1, 1974·Annual Review of Microbiology·P A SRERE, K Mosbach

Citations

Jan 1, 1983·The International Journal of Biochemistry·S Beeckmans, L Kanarek
Jan 1, 1984·The International Journal of Biochemistry·S Beeckmans
Jan 1, 1978·Advances in Enzyme Regulation·C K MathewsG Prem veer Reddy
Nov 7, 1981·British Medical Journal·R Smith

Related Concepts

Citrate (si)-Synthase
Diffusion
Oxo-Acid-Lyases
Malate Dehydrogenase

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