Aspergillus Biofilm In Vitro and In Vivo

Microbiology Spectrum
A Beauvais, J P Latgé

Abstract

In vivo, Aspergillus fumigatus grows as a typical biofilm with hyphae covered by an extracellular matrix (ECM) composed of polysaccharides, galactomannan, and galactosaminogalactan. α1,3 glucans and melanin are also constitutive of the ECM in aspergilloma but not in invasive aspergillosis. In vitro, two biofilm models were established to mimic the in vivo situation. The first model (model 1) uses submerged liquid conditions and is characterized by slow growth, while the second model (model 2) uses agar medium and aerial conditions and is characterized by rapid growth. The composition of the ECM was studied only in the second model and has been shown to be composed of galactomannan, galactosaminogalactan (GAG), and α1,3 glucans, melanin, antigens, and hydrophobins. The presence of extracellular DNA was detected in model 1 biofilm but not in model 2. Transcriptomic analysis employing both biofilm models showed upregulation of genes coding for proteins involved in the biosynthesis of secondary metabolites, adhesion, and drug resistance. However, most data on A. fumigatus biofilms have been obtained in vitro and should be confirmed using in vivo animal models. There is a need for new therapeutic antibiofilm strategies that focus on...Continue Reading

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Citations

Sep 8, 2016·Cellular Microbiology·Christine HenryJean-Paul Latgé
Oct 21, 2016·BMC Microbiology·Alejandra Itzel González-RamírezMaría Angeles Martínez-Rivera
Nov 22, 2016·Pediatric Pulmonology·Abigail C CowleyAlexander Horsley
Jan 27, 2018·Journal of Fungi·Hasan NazikDavid A Stevens
Jul 25, 2018·Critical Reviews in Biotechnology·Digar SinghChoong Hwan Lee
May 3, 2019·Biophysics Reviews·Nirukshan ShanmugamMargaret Sunde
Sep 19, 2017·Nature Reviews. Microbiology·Frank L van de VeerdonkJean-Paul Latgé
Apr 30, 2020·Investigative Ophthalmology & Visual Science·Lu ZhanGuiqiu Zhao
Aug 6, 2020·Frontiers in Microbiology·Larissa Naiara Carvalho GonçalvesAna Marisa Fusco-Almeida
Jul 30, 2020·Journal of Fungi·Hasan NazikDavid A Stevens
Jun 9, 2017·Medical Mycology·Raimunda Sâmia Nogueira BrilhanteJosé Júlio Costa Sidrim
Nov 2, 2019·MSphere·Suresh AmbatiRichard B Meagher
Jan 30, 2018·Frontiers in Immunology·John F KernienJeniel E Nett
Oct 13, 2020·Current Clinical Microbiology Reports·Krystal Y Chung, Jessica C S Brown
Oct 31, 2020·Journal of Fungi·Claudia Rodriguez-de la NovalAllan J Guimarães
Aug 7, 2021·Critical Reviews in Microbiology·Maryam RoudbaryCélia F Rodrigues
Nov 2, 2021·Frontiers in Cellular and Infection Microbiology·Joo-Yeon LimHee-Moon Park

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