Abstract
The genes of two α-L-arabinofuranosidases (AbfI and II) from family GH 62 have been identified in the genome of Aspergillus fumigatus wmo. Both genes have been expressed in Pichia pastoris and the enzymes have been purified and characterized. AbfI is composed of 999 bp, does not contain introns and codes for a protein (ABFI) of 332 amino acid residues. abfII has 1246 bp, including an intron of 51 bp; the protein ABFII has 396 amino acid residues; it includes a family 1 carbohydrate-binding module (CBM) in the N-terminal region, followed by a catalytic module. The sequence of ABFI and the catalytic module of ABFII show a 79 % identity. Both enzymes are active on p-nitrophenyl α-L-arabinofuranoside (pNPAra) with KM of 94.2 and 3.9 mM for ABFI and II, respectively. Optimal temperature for ABFI is 37 °C and for ABFII 42 °C, while the pH optimum is about 4.5 to 5 for both enzymes. ABFII shows a higher thermostability. When assayed using natural substrates, both show higher activity over rye arabinoxylan as compared to wheat arabinoxylan. ABFII only is active on sugar beet pulp arabinan and both are inactive towards debranched arabinan. The higher thermostability, higher affinity for pNPAra and wider activity over natural substrates ...Continue Reading
References
Jun 1, 1996·The Biochemical Journal·B Henrissat, A Bairoch
Nov 1, 1996·Metabolism: Clinical and Experimental·K SeriS Inoue
Feb 18, 1998·Molecular Microbiology·N N van PeijL H de Graaff
Mar 5, 1999·International Journal of Neural Systems·H NielsenG von Heijne
Aug 5, 2000·Fungal Genetics and Biology : FG & B·S H Denison
Dec 31, 2005·Journal of Industrial Microbiology & Biotechnology·Mondher Th Numan, Narayan B Bhosle
Jul 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefan BauerChris R Somerville
Sep 8, 2007·The Protein Journal·Dominic Wong
May 17, 2008·Applied Microbiology and Biotechnology·Dominic W S WongSarah B Batt
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Nov 13, 2009·Applied Microbiology and Biotechnology·Daniel GuillénRomina Rodríguez-Sanoja
Aug 11, 2010·Glycobiology·Byung H ParkEdward C Uberbacher
Nov 3, 2010·Applied and Environmental Microbiology·Marie CouturierJean-Guy Berrin
Dec 25, 2010·Applied Microbiology and Biotechnology·Tatsuji SakamotoNaoya Kasai
Feb 11, 2011·Bioscience, Biotechnology, and Biochemistry·Kohsuke HashimotoKeiji Hasumi
May 31, 2013·Fungal Biology·Lorena RosaJaime Eyzaguirre
Sep 17, 2013·Enzyme and Microbial Technology·Marion De La MareJean M Francois
Jan 8, 2014·The Journal of Biological Chemistry·Béatrice SiguierClaire Dumon
Feb 1, 2014·The Journal of Biological Chemistry·Tomoko MaeharaSatoshi Kaneko
Jun 22, 2014·Applied and Environmental Microbiology·Weijun WangEmma R Master
Oct 1, 2014·Microbial Biotechnology·Amrit Pal KaurAlexei Savchenko
Citations
Sep 4, 2016·Applied Biochemistry and Biotechnology·Takashi TonozukaMakoto Yoshida
Apr 4, 2018·AMB Express·Yanbo HuYifa Zhou
Mar 25, 2019·Applied Microbiology and Biotechnology·Alei GengJianzhong Sun
Sep 1, 2020·Biochimica Et Biophysica Acta. Proteins and Proteomics·Suelen CamargoJoão R C Muniz