Aspergillus oryzae pathways that convert phenylalanine into the flavor volatile 2-phenylethanol

Fungal Genetics and Biology : FG & B
Shunsuke MasuoNaoki Takaya

Abstract

The filamentous fungus Aspergillus oryzae RIB40 produced 2-phenylethanol (PE) when cultured in minimum medium containing l-phenylalanine as a sole source of nitrogen. The fungus accumulated less PE in the absence of l-phenylalanine, indicating that it converted l-phenylalanine to PE. The PE production associated with fungal glucose consumption was repressed by exogenous ammonium, indicating that nitrogen-metabolite repression controls the pathway that produces PE. We identified the A. oryzae ppdA gene that is expressed at high levels in the presence of exogenous l-phenylalanine and its encoded protein was an active phenylpyruvate decarboxylase. The fungal genome encodes predicted aminotransferases of phenylalanine and PE dehydrogenases, which, together with PpdA, are likely to constitute an Erlich pathway similar to that in Saccharomyces cerevisiae that produces PE. We also identified an A. oryzae aromatic amino acid decarboxylase (AadA) that converted l-phenylalanine to phenylethylamine (PEA), and phenylalanine-inducible PEA oxidase activity in fungal cell extracts, and found that both constitute an alternative pathway through which PEA generates PE. Incubating fungal cultures with l-[(2)H8] phenylalanine to distinguish PE pro...Continue Reading

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Citations

Aug 13, 2015·Current Opinion in Biotechnology·Brian ThompsonDavid R Nielsen
May 12, 2016·Scientific Reports·Shunsuke MasuoNaoki Takaya
Dec 21, 2018·Critical Reviews in Biotechnology·Xiujuan QianFengxue Xin
Jun 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min Kyung Park, Young-Suk Kim
Oct 8, 2018·Applied Microbiology and Biotechnology·Oscar Martínez-AvilaRaquel Barrena
Jan 22, 2019·Frontiers in Bioengineering and Biotechnology·Behrouz Mohammadi NargesiJung-Won Youn
Jul 2, 2020·Journal of Agricultural and Food Chemistry·Jinbin LiuYujie Cai
Mar 22, 2018·Journal of Agricultural and Food Chemistry·Jinbin LiuYujie Cai
Apr 13, 2019·Journal of Agricultural and Food Chemistry·Jeehye SungYu Wang
Nov 19, 2021·Journal of Agricultural and Food Chemistry·Joon Hyuk SuhYu Wang

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