Aspergillus oryzae type III polyketide synthase CsyA is involved in the biosynthesis of 3,5-dihydroxybenzoic acid

Bioorganic & Medicinal Chemistry Letters
Yasuyo SeshimeIsao Fujii

Abstract

As a novel superfamily of type III polyketide synthases in microbes, four genes csyA, csyB, csyC, and csyD, were found in the genome of Aspergillus oryzae, an industrially important filamentous fungus. In order to analyze their functions, we carried out the overexpression of csyA under the control of alpha-amylase promoter in A. oryzae and identified 3,5-dihydroxybenzoic acid (DHBA) as the major product. Feeding experiments using (13)C-labeled acetates confirmed that the acetate labeling pattern of DHBA coincided with that of orcinol derived from orsellinic acid, a polyketide formed by the condensation and cyclization of four acetate units. Further oxidation of methyl group of orcinol by the host fungus could lead to the production of DHBA. Comparative molecular modeling of CsyA with the crystal structure of Neurospora crassa 2'-oxoalkylresorcylic acid synthase indicated that CsyA cavity size can only accept short-chain acyl starter and tetraketide formation. Thus, CsyA is considered to be a tetraketide alkyl-resorcinol/resorcylic acid synthase.

References

Dec 24, 2005·Nature·Masayuki MachidaHisashi Kikuchi
Oct 31, 2008·Chembiochem : a European Journal of Chemical Biology·Silke C WenzelRolf Müller
Feb 23, 2010·Molecular BioSystems·James F SanchezClay C C Wang

❮ Previous
Next ❯

Citations

Jun 11, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Dengfeng YangIkuro Abe
Apr 26, 2011·Annual Review of Phytopathology·Saori Amaike, Nancy P Keller
Oct 20, 2012·Fungal Genetics and Biology : FG & B·Gerald LacknerDirk Hoffmeister
May 7, 2011·Natural Product Reports·Tohru DairiIsao Fujii
Jun 11, 2020·Biomolecules·Egle ValancieneNaglis Malys
Jan 10, 2012·Natural Product Reports·James F SanchezClay C C Wang
Jan 12, 2013·Chembiochem : a European Journal of Chemical Biology·Asifa MunawarColin M Lazarus
Sep 4, 2014·Natural Product Reports·Makoto HashimotoIsao Fujii
Nov 20, 2016·Chembiochem : a European Journal of Chemical Biology·Yugo ShimizuSusumu Goto
Feb 11, 2020·Frontiers in Microbiology·Jorge Carlos Navarro-Muñoz, Jérôme Collemare
Mar 20, 2021·Genome Biology and Evolution·Joachim Steen LarsenBrett Anthony Neilan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.