Aspirin Breaks the Crosstalk between 3T3-L1 Adipocytes and 4T1 Breast Cancer Cells by Regulating Cytokine Production

PloS One
Chia-Chien Hsieh, Yu-Shan Huang

Abstract

Breast cancer is one of the most common cancers in women worldwide. The obesity process is normally accompanied by chronic, low-grade inflammation. Infiltration by inflammatory cytokines and immune cells provides a favorable microenvironment for tumor growth, migration, and metastasis. Epidemiological evidence has shown that aspirin is an effective agent against several types of cancer. The aim of this study is to investigate the anti-inflammatory and anti-cancer effects of aspirin on 3T3-L1 adipocytes, 4T1 murine breast cancer cells, and their crosstalk. The results showed that aspirin treatment inhibited differentiation and lipid accumulation by 3T3-L1 preadipocytes, and decreased the secretion of the inflammatory adipokine MCP-1 after stimulation with tumor necrosis factor (TNF)-α or conditioned medium from RAW264.7 cells. In 4T1 cells, treatment with aspirin decreased cell viability and migration, possibly by suppressing MCP-1 and VEGF secretion. Subsequently, culture of 4T1 cells in 3T3-L1 adipocyte-conditioned medium (Ad-CM) and co-culture of 3T3-L1 and 4T1 cells using a transwell plate were performed to clarify the relationship between these two cell lines. Aspirin exerted its inhibitory effects in the transwell co-cultu...Continue Reading

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Citations

Apr 14, 2018·Tissue Engineering. Part a·Robert D HumeChristine J Watson
Feb 3, 2018·Investigational New Drugs·Maria Eduarda Azambuja AmaralMaria Martha Campos
Oct 4, 2017·Frontiers in Immunology·Luís Henrique CorrêaKelly Grace Magalhães
Dec 17, 2016·International Journal of Molecular Sciences·Chia-Chien HsiehYu-Shan Huang
Nov 1, 2018·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Mollie E BarnardShelley S Tworoger
Oct 23, 2021·Molecular Medicine Reports·Huihui Yao, Songbing He

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Methods Mentioned

BETA
ELISA
scraping

Software Mentioned

Statistical Product and Service Solutions ( SPSS )

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