Aspirin induces apoptosis in YD-8 human oral squamous carcinoma cells through activation of caspases, down-regulation of Mcl-1, and inactivation of ERK-1/2 and AKT

Toxicology in Vitro : an International Journal Published in Association with BIBRA
In-Sook ParkByeong-Churl Jang

Abstract

NSAIDs and COX-2 inhibitors show anti-cancer activities in many cancer cells. In this study, we investigated the effects of NSAIDs (aspirin or indomethacin) and COX-2 inhibitor (NS-398) on growth of YD-8 human oral squamous carcinoma cells. Interestingly, among drugs tested, aspirin showed strongest inhibitory effects on viability and survival of YD-8 cells. Profoundly, aspirin treatment resulted in severe cell shrinkage and nuclear DNA fragmentation in YD-8 cells, suggesting the aspirin-induced apoptosis in YD-8 cells. Data of Western blot further demonstrated that aspirin treatment caused activation of caspases, down-regulation of Mcl-1 protein, dephosphorylation of ERK-1/2 and AKT, and also IkappaB-alpha proteolysis-dependent NF-kappaB activation in YD-8 cells. Aspirin, however, had no effect on expressions of Bcl-2, XIAP, and HIAP-1 in YD-8 cells. Importantly, pretreatment with z-VAD-fmk, a pan-caspase inhibitor blocked the aspirin-induced apoptosis and Mcl-1 down-regulation in YD-8 cells. These findings collectively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activation of caspases, caspase-dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of NF-kap...Continue Reading

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Citations

Feb 22, 2011·European Journal of Medicinal Chemistry·Matthias ScholzEvamarie Hey-Hawkins
Apr 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Stamatios TheocharisXavier Sastre-Garau
Apr 25, 2013·Biochemical and Biophysical Research Communications·Gongquan LiXuexiang Ye
Oct 25, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chang-Wook JungJong-Wook Park
Jun 22, 2013·Acta Biochimica Et Biophysica Sinica·Hanju HuaLisong Teng
Jan 20, 2018·Biotechnology Letters·Ting-Ting XiaoYong Li
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Jun 18, 2020·International Journal of Molecular Sciences·Cheng-Yi ChangChun-Jung Chen
Dec 4, 2014·Molecular and Clinical Oncology·Lucia HiľovskáPeter Fedoročko
Mar 9, 2021·Head & Neck·Constanza Saka-HerránJosé López-López

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