Aspirin inhibits endothelial cell activation induced by antiphospholipid antibodies

Journal of Thrombosis and Haemostasis : JTH
Sylvie Dunoyer-GeindrePhilippe de Moerloose

Abstract

Antiphospholipid antibodies (APLA) have been shown to activate endothelial cells (EC) in vitro, as documented by an increased expression of tissue factor as well as leukocyte adhesion molecules such as intercellular adhesion molecule-1, vascular cell adhesion molecule (VCAM)-1 and E-selectin. Currently, treatment of patients with the antiphospholipid syndrome includes aspirin, particularly for women with recurrent fetal loss. The present study was undertaken to investigate whether aspirin interferes with EC activation induced by APLA in vitro. IgG from 14 patients with APLA, and suffering from thrombotic complications and/or pregnancy morbidity, and control IgG were tested for their ability to modify the expression of VCAM-1 in human umbilical vein endothelial cells. VCAM-1 antigen was measured by flow cytometry and its mRNA by quantitative reverse transcriptase-polymerase chain reaction. Incubation of EC with IgG from most of the patients led to a higher VCAM-1 expression compared with incubation with control IgG. The effect of aspirin was studied for the eight IgG samples that induced a more than 50% increase in VCAM-1. Aspirin (10 mm) treatment of the cells significantly reduced the VCAM-1 response to these APLA. Our results...Continue Reading

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Citations

Jan 20, 2006·Journal of Neurology, Neurosurgery, and Psychiatry·J A OatesN J Gutowski
Jul 16, 2014·Seminars in Oncology·Lucy A Coupland, Christopher R Parish
Jan 1, 2008·Expert Review of Clinical Immunology·Paul R J AmesAnnamaria Margarita
Oct 31, 2007·Autoimmunity Reviews·Cristina C BeliznaYehuda Shoenfeld
Mar 1, 2006·Rheumatic Diseases Clinics of North America·Doruk Erkan, Michael D Lockshin
Feb 8, 2006·Thrombosis Research·M PaccalinP Dighiero
Sep 2, 2014·BioMed Research International·Jenny Amaya-AmayaAdriana Rojas-Villarraga

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Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.