Aspirin rectifies calcium homeostasis, decreases reactive oxygen species, and increases NO production in high glucose-exposed human endothelial cells

Journal of Diabetes and Its Complications
Elena DragomirM Simionescu

Abstract

Aspirin's pharmacological action is mainly related to its property to inhibit prostaglandin synthesis; apart from this, aspirin has some beneficial side effects that are not completely understood, yet. Since aspirin possesses antioxidant properties and antioxidants prevent high d-glucose enhanced endothelial [Ca(2+)](i), we questioned whether aspirin also has an effect on this process as well as on high-glucose-impaired nitric oxide (NO) production. For these purposes, human endothelial cells (HECs) were cultured in normal concentration (5 mM) glucose (NG) or high concentration (33 mM) glucose (HG) and after confluence, exposed for 48 h to HG in the absence or presence of 1 mM aspirin. Then, the [Ca(2+)](i) was measured fluorimetrically using fura-2, NO production was determined by Griess reaction, superoxide anions (O(2)) was evaluated by ferricytochrome c reduction, the intracellular reactive oxygen species (ROS) were evaluated by fluorimetry, and the levels of protein kinase C (PKC) by Western blot. The results showed that HECs exposed to HG displayed: (i) increased [Ca(2+)](i); (ii) enhanced O(2) release; (iii) augmented level of intracellular ROS; and (iv) PKC translocation to the membrane fraction. By comparison, exposure...Continue Reading

References

May 14, 1992·European Journal of Pharmacology·K HishikawaR Kato
Dec 1, 1992·Free Radical Biology & Medicine·A GhiselliA Ferro-Luzzi
Jul 1, 1992·The American Journal of Physiology·P Langenstroer, G M Pieper
Dec 15, 1991·The New England Journal of Medicine·M J ThunC W Heath
Jan 1, 1991·Free Radical Biology & Medicine·S P WolffJ V Hunt
May 1, 1991·The Journal of Clinical Investigation·B TesfamariamR A Cohen
Dec 31, 1990·Biochemical and Biophysical Research Communications·C J MullarkeyM Brownlee
Jan 1, 1990·Annual Review of Physiology·C O Brostrom, M A Brostrom
Oct 1, 1988·Hypertension·S MoncadaE A Higgs
Jan 1, 1988·Free Radical Biology & Medicine·L W Oberley
May 19, 1995·Chemico-biological Interactions·G L Squadrito, W A Pryor
Aug 12, 1994·Science·E Kopp, S Ghosh
Jan 28, 1993·Nature·M J Berridge
Nov 18, 1998·Current Opinion in Nephrology and Hypertension·M MurphyC Godson
Mar 12, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·H M HondaJ A Berliner
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·I M ConboyP P Jones
Sep 4, 1999·Endothelium : Journal of Endothelial Cell Research·I ManduteanuM Simionescu
Feb 7, 2001·Circulation Research·U HinkT Munzel
Jun 28, 2001·Clinical and Diagnostic Laboratory Immunology·M E EremeevaD J Silverman
Feb 19, 2002·Kidney International·Mark A CatherwoodElisabeth R Trimble
May 22, 2002·The Journal of Clinical Investigation·Ripudaman S HundalGerald I Shulman

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Citations

Feb 10, 2007·Kidney International·S M HarwoodM M Yaqoob
Mar 23, 2005·Medical Hypotheses·Christian MurrDietmar Fuchs
Jul 26, 2005·Biochemical and Biophysical Research Communications·Stefanie M Bode-BögerFortunato Scalera
Sep 3, 2016·International Journal of Cardiology·Rajeshwary GhoshAldrin V Gomes
Oct 12, 2011·International Archives of Allergy and Immunology·Daniela ZaknunDietmar Fuchs
Jun 17, 2005·Diabetes/metabolism Research and Reviews
Jan 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Hélène BulckaenFrançois Puisieux
Jul 21, 2006·Metabolic Brain Disease·H MaharajS Daya

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