Assay based on electrical impedance spectroscopy to discriminate between normal and cancerous mammalian cells

Physical Review. E
Fabián Eduardo GianaMariela Inés Bellotti

Abstract

In this work we present an assay to discriminate between normal and cancerous cells. The method is based on the measurement of electrical impedance spectra of in vitro cell cultures. We developed a protocol consisting on four consecutive measurement phases, each of them designed to obtain different information about the cell cultures. Through the analysis of the measured data, 26 characteristic features were obtained for both cell types. From the complete set of features, we selected the most relevant in terms of their discriminant capacity by means of conventional statistical tests. A linear discriminant analysis was then carried out on the selected features, allowing the classification of the samples in normal or cancerous with 4.5% of false positives and no false negatives.

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I Giaever, C R Keese
Feb 1, 1986·IEEE Transactions on Bio-medical Engineering·I Giaever, C R Keese
Apr 1, 1974·Journal of the National Cancer Institute·R B Owens
Jan 30, 2004·Proceedings of the National Academy of Sciences of the United States of America·Charles R KeeseIvar Giaever
Dec 21, 2004·IEEE Transactions on Bio-medical Engineering·Peter AbergStig Ollmar
Dec 13, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·E UrdapilletaF J Bonetto
Nov 13, 2007·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·D C LoveladyD A Rabson
Jan 4, 2008·The Journal of Clinical Investigation·Enrique ZudaireFrank Cuttitta
Apr 10, 2009·Cell Biology International·Giljun ParkTim E Sparer
Mar 23, 2010·Biosensors & Bioelectronics·L Renea AriasLiju Yang
Feb 24, 2012·Journal of Laboratory Automation·Reza RiahiPak Kin Wong

❮ Previous
Next ❯

Citations

Sep 4, 2020·Micromachines·Lexi L CrowellTayloria N G Adams

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
C S Galasko
Netherlands Heart Journal : Monthly Journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation
W J van der Giessen
© 2022 Meta ULC. All rights reserved