PMID: 7513021Jan 1, 1994Paper

Assay for prostate specific antigen (PSA): problems and possible solutions

Journal of Clinical Laboratory Analysis
J T Wu

Abstract

The absolute tissue specificity of prostate specific antigen (PSA) allows the use of PSA test not only for detecting recurrence or metastasis at an early stage after radical prostatectomy but also for screening prostate cancer if combined with digital rectal examination. There is also a need to improve the current PSA test to better differentiate between prostate cancer and benign prostate hyperplasia (BPH). Because of these clinical applications, a much greater demand was placed on PSA test for extra sensitivity, accuracy, and precision even within the normal PSA concentration range. However, the current commercial assay kits for PSA do not provide correct PSA values. Many factors contributing to the problem include the specificity of the anti-PSA antibodies, the composition of the calibrator, the PSA values assigned to the calibrator, the PSA isoform used for anti-PSA antibody preparation, the test design, and the composition of the diluent. Most problems were derived from the failure of realizing earlier that the majority of the PSA exists in serum not as free PSA but as complexes with protease inhibitors. Other problems, such as constantly changing composition of various forms of PSA in serum specimens, and different cleara...Continue Reading

References

Apr 22, 1992·JAMA : the Journal of the American Medical Association·H B CarterP C Walsh
Apr 25, 1991·The New England Journal of Medicine·W J CatalonaG L Andriole
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K W WattR Loor
Apr 1, 1988·The Journal of Urology·G L HortinJ H Ladenson
Jan 1, 1982·Cytogenetics and Cell Genetics·W W Nichols
Apr 27, 1984·Science·H Neurath
Sep 17, 1984·Biochemical and Biophysical Research Communications·Y BanT M Chu

❮ Previous
Next ❯

Citations

Jan 19, 2000·Clinical Biochemistry·B G BlijenbergF H Schröder
Jul 27, 1999·Japanese Journal of Clinical Oncology·M KuriyamaY Ban
Nov 6, 1998·Clinical Chemistry and Laboratory Medicine : CCLM·R Daher, M Beaini
Jul 27, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·B G BlijenbergF H Schröder
May 1, 1997·The Urologic Clinics of North America·R L Vessella, P H Lange
Nov 28, 2001·Protein Expression and Purification·Y WangD S Wishart
Oct 6, 1998·Critical Reviews in Clinical Laboratory Sciences·H G RittenhouseA W Partin
Jun 1, 1997·The Prostate·X GaoK V Honn
Mar 4, 1998·Journal of Clinical Laboratory Analysis·J T WuL Wilson
Jan 1, 1995·Journal of Clinical Laboratory Analysis·J T WuJ T Colemere
Jan 1, 1995·Journal of Clinical Laboratory Analysis·J T WuR Stephenson
Jan 1, 1995·Journal of Clinical Laboratory Analysis·J T Wu, L W Wilson
Aug 4, 2005·Prostate Cancer and Prostatic Diseases·J SpicerH Pandha
Jan 1, 1995·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·C H BangmaF H Schröder
Nov 1, 1996·Annals of Clinical Biochemistry·M J Duffy
May 8, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·K C ChadhaE Sulkowski
Jul 21, 2021·Emerging Topics in Life Sciences·Alexander Gray

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.