Assay of flucytosine (5-fluorocytosine) in human plasma by high-pressure liquid chromatography.

Antimicrobial Agents and Chemotherapy
R W BuryH M Miles

Abstract

The use of the antifungal agent flucytosine has been associated with dose-related hepatotoxicity and bone marrow depression, and measurement of plasma concentrations is useful in determining dosage schedules. The present study describes a rapid, simple, and sensitive high-pressure liquid chromatographic analysis of plasma flucytosine which may be completed within 30 min of receipt of a plasma sample. Sample preparation is minimal and merely involves precipitation of plasma protein before injection onto a reverse-phase column. The method is accurate and reproducible and yields results comparable to or perhaps slightly superior to those obtained by conventional microbiological assay. The value of the procedure for routine use is enhanced by the freedom from interference by a large range of medications administered concurrently with flucytosine.

References

Apr 1, 1975·Antimicrobial Agents and Chemotherapy·R L Kaspar, D J Drutz
Aug 1, 1975·Journal of Pharmaceutical Sciences·A D BlairR E Cutler
Nov 17, 1973·British Medical Journal·D J SchiavoneJ K Dawborn
Feb 1, 1972·The Journal of Infectious Diseases·D B LouriaM Buse
Aug 1, 1974·The Journal of Infectious Diseases·T EilardP Wahlén
Jan 30, 1971·British Medical Journal·C O RecordD C Speller
Jun 1, 1969·Applied Microbiology·S Shadomy

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Citations

Nov 1, 1994·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·J W Hülsewede
May 7, 2002·Evolution; International Journal of Organic Evolution·Jennifer Yeh

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