PMID: 2125512Nov 1, 1990Paper

Assay of glyceryl trinitrate, isosorbide dinitrate, and their metabolites in plasma by large-bore capillary column gas-liquid chromatography

Biopharmaceutics & Drug Disposition
B P BoothK Nakatsu

Abstract

Two large-bore capillary columns, one with dimethyl polysiloxane (HP-1) as the stationary phase and the other with phenyl (50 per cent) methyl (50 per cent) polysiloxane (DB-17), were used to develop gas-liquid chromatographic (GLC) assays for measuring isosorbide dinitrate (ISDN), glyceryl trinitrate (GTN), and their metabolites. ISDN, isosorbide-2-mononitrate (2-ISMN), and isosorbide-5-mononitrate (5-ISMN) in plasma, ranging in concentration from 1 to 300 nM, and GTN, glyceryl-1,2-dinitrate (1,2-GDN), and glyceryl-1,3-dinitrate (1,3-GDN), ranging in concentration from 3 to 60 nM in plasma, were analysed on both columns. GLC analysis yielded baseline resolution of the analytes. The method using the dimethyl polysiloxane column gave a lower limit of detectability for GTN of 0.75 nM (signal/noise (s/n) = 2), and the procedure using the phenyl-methyl column provided a lower limit of detectability for ISDN of 81 pM (s/n = 2). The large-bore column GLC procedures exhibited shorter retention times for both ISDN and GTN than those previously reported for capillary-column assays. The chromatographic resolution of analytes and column efficiency of the large-bore capillary columns were comparable to the results previously found using ca...Continue Reading

References

Mar 1, 1979·Circulation·P W ArmstrongG S Marks
Jan 1, 1987·Drugs·H JaegerZ B Salama
Jul 1, 1988·Clinical Pharmacokinetics·U Thadani, T Whitsett
Oct 1, 1980·The American Journal of Cardiology·P W ArmstrongG S Marks
Jun 1, 1981·British Journal of Clinical Pharmacology·H L FungJ O Parker

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Citations

Feb 27, 2003·Clinical Pharmacokinetics·Satoru Hashimoto, Atsuko Kobayashi
Jul 13, 2002·American Journal of Obstetrics and Gynecology·Mark A BustardGraeme N Smith
Nov 14, 2003·Canadian Journal of Physiology and Pharmacology·Mark A BustardGraeme N Smith

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