Assembly, activation, and trafficking of the Fet3p.Ftr1p high affinity iron permease complex in Saccharomyces cerevisiae.

The Journal of Biological Chemistry
Arvinder SinghDaniel J Kosman

Abstract

The high affinity iron uptake complex in the yeast plasma membrane (PM) consists of the ferroxidase, Fet3p, and the ferric iron permease, Ftr1p. We used a combination of yeast two-hybrid analysis, confocal fluorescence microscopy, and fluorescence resonance energy transfer (FRET) quantification to delineate the motifs in the two proteins required for assembly and maturation into an uptake-competent complex. The cytoplasmic, carboxyl-terminal domain of each protein contains a four-residue motif adjacent to the cytoplasm-PM interface that supports an interaction between the proteins. This interaction has been quantified by two-hybrid analysis and is required for assembly and trafficking of the complex to the PM and for the approximately 13% maximum FRET efficiency determined. In contrast, the Fet3p transmembrane domain (TM) can be exchanged with the TM domain from the vacuolar ferroxidase, Fet5p, with no loss of assembly and trafficking. A carboxyl-terminal interaction between the vacuolar proteins, Fet5p and Fth1p, also was quantified. As a measure of the specificity of interaction, no interaction between heterologous ferroxidase permease pairs was observed. Also, whereas FRET was quantified between fluorescent fusions of the co...Continue Reading

References

Jan 1, 1974·Advances in Experimental Medicine and Biology·E Frieden, S Osaki
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S YuanR D Klausner
Jan 20, 1995·The Journal of Biological Chemistry·D M De SilvaJ Kaplan
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·S R Davis-KaplanJ Kaplan
Dec 23, 1999·The Journal of Biological Chemistry·J L Urbanowski, R C Piper
Mar 14, 2000·The Journal of Biological Chemistry·N Branza-NichitaS M Petrescu
Jul 27, 2002·Annual Review of Microbiology·Anthony Van HoJerry Kaplan
Sep 10, 2002·Methods : a Companion to Methods in Enzymology·Mark C Overton, Kendall J Blumer
Nov 7, 2002·Advances in Protein Chemistry·Daniel J Kosman
Feb 27, 2003·Molecular Microbiology·Daniel J Kosman
Apr 10, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Tzu-Pin WangDaniel J Kosman
Sep 5, 2003·The Journal of Biological Chemistry·Xiaoli ShiZhiwu Zhu
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alexander B TaylorP John Hart

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Citations

Nov 22, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Alaina J Terzulli, Daniel J Kosman
Jun 5, 2010·The Journal of Biological Chemistry·Daniel J Kosman
Aug 25, 2009·Eukaryotic Cell·Won Hee JungJames W Kronstad
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Marcus Miethke, Mohamed A Marahiel
Dec 19, 2006·PLoS Computational Biology·Dmitry A RodionovAndrew W B Johnston
Mar 5, 2014·Chemical Reviews·Edward I SolomonLi Tian
Apr 26, 2014·Molecular Plant-microbe Interactions : MPMI·Bradford J CondonB Gillian Turgeon
Apr 12, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Mark R Bleackley, Ross T A Macgillivray
Oct 10, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Daniel J Kosman
Jun 8, 2007·Biochimica Et Biophysica Acta·Brian R KupchakThomas J Lyons
Feb 28, 2018·Metallomics : Integrated Biometal Science·Daniel J Kosman
Jul 28, 2010·Protein Science : a Publication of the Protein Society·Lynn ZieglerDaniel J Kosman
Feb 17, 2018·Scientific Reports·Ángel Vizoso-VázquezM Esperanza Cerdán
Jan 16, 2007·The Journal of Biological Chemistry·Christopher S StojDaniel J Kosman
Sep 24, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Sarah KönigKlaus Groschner
Oct 17, 2019·Biosensors·Michal SkruznyMarc Abella
Jul 14, 2007·The Journal of Biological Chemistry·Devis SinaniJaekwon Lee
Aug 29, 2012·Molecular Microbiology·Timothy J Brickman, Sandra K Armstrong
May 6, 2019·Microbiology·Frédéric Devaux, Antonin Thiébaut
Apr 11, 2007·The Journal of Cell Biology·Todd I StrochlicChristopher G Burd
Apr 27, 2021·Computational and Structural Biotechnology Journal·Cláudia Barata-AntunesSandra Paiva
May 1, 2021·Biomolecules·Marina VolkovaAlbert Guskov
Jul 23, 2021·Molecular Cell·Matthew DomnauerChuankai Zhou
Jul 10, 2014·ACS Chemical Biology·Tatsuya FukushimaKenneth N Raymond
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