Apr 8, 2016

Assembly and Activation of Dynein-Dynactin by the Cargo Adaptor Protein Hook3

BioRxiv : the Preprint Server for Biology
Courtney M Schroeder, Ronald D Vale


Metazoan cytoplasmic dynein moves processively along microtubules with the aid of dynactin and an adaptor protein that joins dynein and dynactin into a stable ternary complex. Here, we have examined how Hook3, a cargo adaptor involved in Golgi and endosome transport, forms a motile dynein-dynactin complex. We show that the conserved Hook domain interacts directly with the dynein light intermediate chain 1 (LIC1). By solving the crystal structure of the Hook domain and using structure-based mutagenesis, we identify two conserved surface residues that are each critical for LIC1 binding. Hook proteins with mutations in these residues fail to form a stable dynein-dynactin complex, revealing a crucial role for LIC1 in this interaction. We also identify a region of Hook3 specifically required for an allosteric activation of processive motility. Our work reveals the structural details of Hook3's interaction with dynein and offers insight into how cargo adaptors form processive dynein-dynactin motor complexes.

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Mentioned in this Paper

Cavity of Golgi Vesicle
DCTN4 protein, human
Dynein Activity
Golgi Apparatus
Cell Motility
Endosome Transport
Complex (molecular entity)
Protein Hook Homolog 3
Dynactin Complex

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