Assembly and optimization of expression of synthetic genes derived from the human elastin repeated motif

Preparative Biochemistry & Biotechnology
Antonella Bandiera

Abstract

Naturally occurring proteins often possess interesting properties that make them attractive for the realization of innovative biomaterials. Repetitive artificial polypeptides have been modeled on the repeated domains of mammalian elastin, retaining and even enhancing their thermally responsive behavior. These protein polymers have been produced by recombinant biotechnology and due to their smart properties show a huge potential for a wide range of biomedical and biotechnological applications. For this reason, production of large quantities of highly purified material is a crucial step. We focused our attention on elastin-derived polypeptides based on the hexapeptidic motif typical of human elastin. Synthetic genes were assembled starting from a monomeric unit, and the different conditions were assayed to optimize the yield of the artificial polypeptides. Optimization of Escherichia coli strain and of the extraction procedure led to significant improvement in expression and recovery of the recombinant products. Electron micrographs of expressing bacteria under optimized conditions showed the accumulation of the recombinant product in the induced cells.

Citations

Jun 10, 2014·Expert Opinion on Drug Delivery·Gianni CiofaniAntonella Bandiera
Oct 23, 2012·Acta Biomaterialia·Gianni CiofaniAntonella Bandiera
Jun 11, 2014·Journal of Biomedical Materials Research. Part a·Francesca BoccafoschiMario Cannas
Aug 2, 2015·Biomaterials·Paola D'AndreaAntonella Bandiera
Jul 15, 2016·Expert Opinion on Drug Delivery·Antonella Bandiera
May 2, 2014·Current Opinion in Biotechnology·Skander ElleucheGarabed Antranikian
Nov 7, 2019·Biotechnology and Bioengineering·Antonella BandieraSabina Passamonti
Dec 10, 2013·Macromolecular Bioscience·Gianni CiofaniAntonella Bandiera
Oct 9, 2012·Macromolecular Bioscience·Betül ÇelebiAntonella Bandiera
Sep 28, 2018·Frontiers in Bioengineering and Biotechnology·Antonella BandieraMaria Letizia Focarete
Oct 30, 2016·Journal of Applied Biomaterials & Functional Materials·Paola D'AndreaAntonella Bandiera
Dec 24, 2013·Biomacromolecules·Antonella BandieraVioletta Borrelli
Aug 26, 2021·Biotechnology and Applied Biochemistry·Antonella BandieraLisa Elviri

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
transmission electron microscopy

Software Mentioned

NEBexpress

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.