Assembly characteristics of flagellar cap protein HAP2 of Salmonella: decamer and pentamer in the pH-sensitive equilibrium

Journal of Molecular Biology
Katsumi ImadaKeiichi Namba

Abstract

The cap of the bacterial flagellum is an oligomeric assembly of HAP2 protein (also called FliD), tightly attached to the tip of the flagellar filament. Flagellar growth does not occur in fliD-deficient mutants because flagellin monomers transported through the central channel of the flagellum leak out without polymerizing at the distal end. The structure of the cap complex is not known yet. An in vitro assembly of HAP2 proteins was found to have a pentagonal shape, while its molecular mass corresponded roughly to that of a dodecamer. To characterize the structure and assembly behavior of the complex formed in vitro in more detail, the stoichiometry of the complex and the association equilibrium have been studied. Crosslinking experiments now clearly show that the HAP2 complex is decameric. The assembly equilibrium is mainly between the monomer and decamer with a minor population of intermediate oligomers involved, and is highly dependent on the solution pH as well as the salt concentration: the fraction of the decamer sharply rises as the pH decreases from 8.5 to 8.0; the physiological concentration of salt partially suppresses the decamer formation. A preferential crosslinking within a pentameric unit together with a bipolar f...Continue Reading

References

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Citations

Feb 13, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K Namba
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Katsumi ImadaKeiichi Namba
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Tohru Minamino, Keiichi Namba
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Saori Maki-YonekuraKeiichi Namba
Mar 8, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Fumio HayashiMasahiro Ishiura
May 6, 2003·Annual Review of Microbiology·Robert M Macnab
Oct 21, 2016·ELife·Sandra PostelEric J Sundberg
Jan 8, 1999·Journal of Molecular Biology·F VondervisztK Namba
Aug 28, 2007·Biochimica Et Biophysica Acta·Dmitry Apel, Michael G Surette
Feb 10, 2017·Journal of Molecular Biology·Wan Seok SongSung-Il Yoon
Dec 14, 2017·Biophysics Reviews·Katsumi Imada
Jun 16, 2004·FEBS Letters·Zoltán DiószeghyFerenc Vonderviszt
May 17, 2008·Nature Reviews. Microbiology·Fabienne F V Chevance, Kelly T Hughes
Nov 30, 2015·Biochemistry. Biokhimii︠a︡·I A OstermanP V Sergiev
Feb 21, 2002·World Journal of Gastroenterology : WJG·W S JiD M Fan

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