Assembly of exogenous fibronectin into type II cell extracellular matrix

The American Journal of Physiology
J W Swisher, D E Rannels

Abstract

Type II pulmonary epithelial cells (T2P) in primary culture assemble a biologically active extracellular matrix (ECM) from endogenously synthesized components, including fibronectin. Fibronectin is a well-recognized attachment protein that mediates cell adhesion, migration, and cytodifferentiation. In some cell types, exogenous fibronectin also is incorporated into ECM. The latter pathway of ECM assembly was thus investigated in T2P. Cells were cultured for 3-days in Dulbecco's modified Eagle's medium (DMEM) with or without 10% fetal calf serum (FCS), a source of exogenous fibronectin. Cell and matrix fractions were harvested on culture days 1, 2, and 3 to determine synthesis of cell and matrix proteins and matrix fibronectin content. During 3 days in DMEM containing 10% FCS, T2P flattened and spread to confluence more rapidly than cells in DMEM; they also produced ECM with higher fibronectin content than did cells in DMEM alone. On culture days 2 and 3, 10% FCS doubled (on average) synthesis of ECM fibronectin; in contrast, ECM fibronectin content increased nearly 10-fold. These observations suggest that cultured type II cells incorporate exogenous fibronectin into newly assembled ECM to a greater extent than the newly synthes...Continue Reading

Citations

Dec 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Margaret A SchwarzMatthew K Lee
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael Koval
Mar 10, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A I Alford, D E Rannels
Feb 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y GuoD E Rannels
Dec 17, 2002·Journal of Cell Science·Elizabeth E RobinsonRamsey A Foty

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