PMID: 8445716Apr 1, 1993Paper

Assembly of functional Sindbis virus RNA replication complexes: requirement for coexpression of P123 and P34

Journal of Virology
J A Lemm, C M Rice

Abstract

A vaccinia virus transient expression system was used to determine which of the Sindbis virus (SIN) proteins and/or polyproteins are necessary for the formation of active replication complexes and, in particular, to analyze the role of nsP4, the putative polymerase, versus P34 in RNA replication. We generated vaccinia virus recombinants in which the cDNA for the entire SIN nonstructural coding region as well as cDNA copies of the individual nonstructural proteins (nsPs) and several intermediate polyproteins were placed downstream of the promoter for T7 RNA polymerase and the encephalomyocarditis virus 5' untranslated region. The proteins expressed by the vaccinia virus recombinants comigrate with authentic proteins synthesized in SIN-infected cells, and the polyproteins appear to be processed to the individual proteins of the correct size. To examine the replication efficiencies of different protein combinations, a vaccinia virus recombinant was designed to express an engineered substrate RNA which could serve as a template for replication and subgenomic mRNA transcription by the SIN nsPs. Expression of the entire SIN nonstructural coding region resulted in the synthesis of high levels of both genomic and subgenomic RNAs derive...Continue Reading

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Citations

Jan 21, 2011·Journal of Virology·Jonathan C RuppRichard W Hardy
Jul 30, 2015·The Journal of General Virology·Jonathan C RuppRichard W Hardy
May 30, 2020·PLoS Neglected Tropical Diseases·Parveen KaurJustin Jang Hann Chu
Aug 7, 2002·Journal of Virology·Cori L FataDorothea L Sawicki
Aug 15, 2003·The Journal of Biological Chemistry·Lidia VasiljevaTero Ahola
Aug 31, 1999·The Journal of General Virology·E ten DamM D Ryan
Feb 14, 2004·Journal of Virology·Eugene V AgapovCharles M Rice
Nov 11, 2020·Viruses·Mathilde LauretiJulio Rodriguez-Andres
Feb 10, 2018·Frontiers in Plant Science·Xiao-Feng ZhangFeng Qu
Feb 10, 2018·Viruses·Farhana Abu Bakar, Lisa F P Ng
Aug 11, 2018·Frontiers in Molecular Biosciences·Anna K BlakneyRobin J Shattock
Sep 30, 2017·Viruses·Lindsay LundbergKylene Kehn-Hall
Jan 1, 1994·Journal of Virology·T RümenapfJ H Strauss
Nov 26, 1997·Journal of Virology·M M Wielgosz, H V Huang

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