PMID: 2109101May 1, 1990Paper

Assembly of gag-beta-galactosidase proteins into retrovirus particles

Journal of Virology
T A JonesE Barklis

Abstract

We studied the expression of beta-galactosidase (beta-gal) and 15 gag-beta-gal fusion proteins in the presence of Moloney murine leukemia virus wild-type core (gag) proteins. Analysis indicated that proteins retaining the amino-terminal portion of gag through the capsid protein-coding region were incorporated into retrovirus particles. Proteins which deleted portions of the capsid protein were assembled into virions at low efficiency, indicating the importance of capsid protein interactions in retrovirus assembly. Fusion proteins which retained the amino-terminal matrix protein of the gag polyprotein but which lacked the capsid protein were released efficiently from cells in a nonviral form. The nonviral form was characterized by a high sedimentation coefficient and a low density, suggestive of membrane vesicles. While beta-gal was present in the cytoplasm of expressing cells, all fusion constructs were associated with cellular membranes. gag-beta-gal proteins which were capable of release from cells demonstrated a two-component immunofluorescence staining pattern consisting of a circle of fluorescence around the nucleus and a punctate pattern of staining throughout the remainder of the cell. Interestingly, fusions within the m...Continue Reading

References

Aug 12, 1976·Nature·M BarbacidS A Aaronson
Jan 13, 1978·Science·D P BolognesiW Schäfer
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S OroszlanR V Gilden
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
May 10, 1985·Science·N G Lipsky, R E Pagano
Feb 1, 1985·Molecular and Cellular Biology·P A Norton, J M Coffin
Jan 1, 1988·Annual Review of Biochemistry·D A TowlerL Glaser
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
Oct 1, 1988·Molecular and Cellular Biology·I DeichaiteM D Resh
Sep 2, 1988·Science·K StrebelM A Martin
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A ReinA M Schultz
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W ReikR Jaenisch
Sep 13, 1985·Science·R E Pagano, R G Sleight
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Nov 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·C C Widnell, J C Unkeless
Oct 15, 1981·Nature·T M ShinnickJ G Sutcliffe
Jan 1, 1983·The Journal of Cell Biology·A L HubbardA Ma
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H FanM Feuerman
Mar 1, 1983·Journal of Virology·M G RothR W Compans
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L E HendersonS Oroszlan

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Citations

May 9, 1998·The Journal of Biological Chemistry·M BouHamdanL X Duan
Apr 25, 2007·Antimicrobial Agents and Chemotherapy·Eric BarklisAyna Alfadhli
Aug 15, 1991·Nature·G Natsoulis, J D Boeke
Mar 3, 1995·The Journal of Biological Chemistry·G YuR L Felsted
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Ono, E O Freed
Jun 24, 2015·Human Gene Therapy·Kristian Alsbjerg Skipper, Jacob Giehm Mikkelsen
Jul 25, 2014·Mobile Genetic Elements·Yujia Cai, Jacob Giehm Mikkelsen
Jan 31, 2021·The Journal of Biological Chemistry·Eric BarklisFikadu G Tafesse
Jul 1, 1994·Journal of Virology·C A Deminie, M Emerman
Mar 1, 1991·Molecular and Cellular Biology·R PetersenE Barklis
Apr 2, 2003·Virology·Melvyn W Yap, Jonathan P Stoye

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