Assembly of glycoprotein-80 adhesion complexes in Dictyostelium. Receptor compartmentalization and oligomerization in membrane rafts

The Journal of Biological Chemistry
T J HarrisC H Siu

Abstract

The phospholipid-anchored membrane glycoprotein (gp)-80 mediates cell-cell adhesion through a homophilic trans-interaction mechanism during Dictyostelium development and is enriched in a Triton X-100-insoluble floating fraction. To elucidate how gp80 adhesion complexes assemble in the plasma membrane, gp80-gp80 and gp80-raft interactions were investigated. A low density raft-like membrane fraction was isolated using a detergent-free method. It was enriched in sterols, the phospholipid-anchored proteins gp80, gp138, and ponticulin, as well as DdCD36 and actin, corresponding to components found in the Triton X-100-insoluble floating fraction. Chemical cross-linking revealed that gp80 oligomers were enriched in the raft-like membrane fraction, implicating stable oligomer-raft interactions. However, gp80 oligomers resisted sterol sequestration and were partially dissociated with Triton X-100, suggesting that compartmentalization in rafts was not solely responsible for their formation. The trans-dimer known to mediate adhesion was identified, but cis-oligomerization predominated and displayed greater accumulation during development. In fact, oligomerization was dependent on the level of gp80 expression and occurred among isolated gp...Continue Reading

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Citations

Oct 19, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Tony J C Harris, Chi-Hung Siu
Aug 24, 2004·Infection and Immunity·Richard C LaughlinLesly A Temesvari
Nov 25, 2004·Seminars in Cell & Developmental Biology·Chi-Hung SiuEstella Wong
Sep 16, 2004·Biological Reviews of the Cambridge Philosophical Society·Vivienne M Bowers-MorrowKeith L Williams
Sep 24, 2004·Journal of Theoretical Biology·John C Dallon, Hans G Othmer
Jan 21, 2018·Journal of Cellular Biochemistry·Sarah AlamerRobert E Gundersen
Apr 23, 2004·American Journal of Physiology. Cell Physiology·M M SedenskyP G Morgan
Aug 17, 2005·Traffic·Isabel C Morrow, Robert G Parton
Jan 23, 2018·Frontiers in Immunology·Joe Dan DunnThierry Soldati

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