PMID: 9529374May 16, 1998Paper

Assembly of lampbrush chromosomes from sperm chromatin

Molecular Biology of the Cell
J G Gall, C Murphy

Abstract

We have examined the behavior of demembranated sperm heads when injected into the germinal vesicle (GV) of amphibian oocytes. Xenopus sperm heads injected into Xenopus GVs swelled immediately and within hours began to stain with an antibody against RNA polymerase II (Pol II). Over time each sperm head became a loose mass of chromosome-like threads, which by 24-48 h resolved into individually recognizable lampbrush chromosomes (LBCs). Although LBCs derived from sperm are unreplicated single chromatids, their morphology and immunofluorescent staining properties were strikingly similar to those of the endogenous lampbrush bivalents. They displayed typical transcriptionally active loops extending from an axis of condensed chromomeres, as well as locus-specific "landmarks. " Experiments with [3H]GTP and actinomycin D demonstrated that transcription was not necessary for the initial swelling of the sperm heads and acquisition of Pol II but was required for maintenance of the lampbrush loops. Splicing was not required at any stage during formation of sperm LBCs. When Xenopus sperm heads were injected into GVs of the newt Notophthalmus, the resulting sperm LBCs displayed very long loops with pronounced Pol II axes, like those of the en...Continue Reading

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Citations

Oct 20, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Alla KrasikovaElena Gaginskaya
Dec 21, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Garry T Morgan
Mar 25, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Christopher AustinMichel Bellini
Sep 8, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Herbert C Macgregor
Dec 25, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Joseph G Gall
Jan 9, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Ji-Long Liu, Joseph G Gall
Dec 20, 2002·Journal of Structural Biology·Olivia DoyleJoseph G Gall
Apr 20, 2013·Chemical Reviews·Argyris Papantonis, Peter R Cook
Mar 13, 2003·Molecular Biology of the Cell·Abigail J SmithGarry T Morgan
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Christopher L Woodcock, Rajarshi P Ghosh
Jan 31, 2007·Molecular and Cellular Biology·Brent BeendersMichel Bellini
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J G Gall
Feb 7, 2007·Trends in Genetics : TIG·Davide MarenduzzoPeter R Cook
Apr 1, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·May Penrad-MobayedCaroline Perrin
Dec 7, 2013·Angewandte Chemie·John B Gurdon
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Sep 28, 2020·Experimental Cell Research·J B GurdonH M Barbosa Triana
May 30, 2013·Development·J B Gurdon

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