Assembly of lipase and P450 fatty acid decarboxylase to constitute a novel biosynthetic pathway for production of 1-alkenes from renewable triacylglycerols and oils

Biotechnology for Biofuels
Jinyong YanShengying Li

Abstract

Biogenic hydrocarbons (biohydrocarbons) are broadly accepted to be the ideal 'drop-in' biofuel alternative to petroleum-based fuels due to their highly similar chemical composition and physical characteristics. The biological production of aliphatic hydrocarbons is largely dependent on engineering of the complicated enzymatic network surrounding fatty acid biosynthesis. In this work, we developed a novel system for bioproduction of terminal fatty alkenes (1-alkenes) from renewable and low-cost triacylglycerols (TAGs) based on the lipase hydrolysis coupled to the P450 catalyzed decarboxylation. This artificial biosynthetic pathway was constituted using both cell-free systems including purified enzymes or cell-free extracts, and cell-based systems including mixed resting cells or growing cells. The issues of high cost of fatty acid feedstock and complicated biosynthesis network were addressed by replacement of the de novo biosynthesized fatty acids with the fed cheap TAGs. This recombinant tandem enzymatic pathway consisting of the Thermomyces lanuginosus lipase (Tll) and the P450 fatty acid decarboxylase OleTJE resulted in the production of 1-alkenes from purified TAGs or natural oils with 6.7 to 46.0% yields. Since this novel h...Continue Reading

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Citations

Nov 9, 2016·Biotechnology for Biofuels·James C du Preez
Jan 6, 2017·The Journal of Biological Chemistry·Sarah MatthewsAndrew W Munro
Nov 12, 2019·Organic & Biomolecular Chemistry·Shiqing Zhang, Yongjun Liu
Jul 14, 2020·Chembiochem : a European Journal of Chemical Biology·Daniel BauerVolker Sieber
May 22, 2020·Frontiers in Bioengineering and Biotechnology·Letícia L RadeLeticia M Zanphorlin
Dec 24, 2019·Journal of Biotechnology·Juthamas JaroensukPimchai Chaiyen
Jul 26, 2021·Biochemical and Biophysical Research Communications·Hannah L Wapshott-Stehli, Amy M Grunden
Sep 30, 2021·Chemical Communications : Chem Comm·Yi ZhouUwe T Bornscheuer

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Datasets Mentioned

BETA
AF054513.1

Methods Mentioned

BETA
Protein Assay
PCR

Software Mentioned

GenScript

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