Assembly of NADPH oxidase in human neutrophils is modulated by the opacity-associated protein expression State of Neisseria gonorrhoeae

Infection and Immunity
Asya SmirnovAlison K Criss

Abstract

Neisseria gonorrhoeae (the gonococcus, Gc) triggers a potent inflammatory response and recruitment of neutrophils to the site of infection. Gc survives exposure to neutrophils despite these cells' antimicrobial products, such as reactive oxygen species (ROS). ROS production in neutrophils is initiated by NADPH oxidase, which converts oxygen into superoxide. The subunits of NADPH oxidase are spatially separated between granules (gp91(phox)/p22(phox)) and the cytoplasm (p47(phox), p67(phox), and p40(phox)). Activation of neutrophils promotes the coassembly of NADPH oxidase subunits at phagosome and/or plasma membranes. While Gc-expressing opacity-associated (Opa) proteins can induce neutrophils to produce ROS, Opa-negative (Opa-) Gc does not stimulate neutrophil ROS production. Using constitutively Opa- and OpaD-positive (OpaD+) Gc bacteria in strain FA1090, we now show that the difference in ROS production levels in primary human neutrophils between these backgrounds can be attributed to differential assembly of NADPH oxidase. Neutrophils infected with Opa- Gc showed limited translocation of NADPH oxidase cytoplasmic subunits to cellular membranes, including the bacterial phagosome. In contrast, these subunits rapidly translocat...Continue Reading

References

Feb 1, 1980·Disease-a-month : DM·P J Wiesner, S E Thompson
Jan 1, 1980·Canadian Journal of Microbiology·S A Morse, L Bartenstein
Jun 1, 1994·The Journal of Clinical Investigation·H S SeifertJ G Cannon
Mar 1, 1994·The Journal of Experimental Medicine·A E JerseJ G Cannon
Apr 1, 1997·Molecular and Cellular Biology·I de MendezT L Leto
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P M DangA Périanin
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Kim, M C Dinauer
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carolyn R HoyalBernard M Babior
May 7, 2003·Cell·Yvonne GroempingKatrin Rittinger
Jun 1, 1963·Journal of Bacteriology·D S KELLOGGD I PIRKLE
Jan 13, 2004·Nature Medicine·Ji-Jing YanDong-Keun Song
Jul 9, 2004·Journal of Leukocyte Biology·Mark T Quinn, Katherine A Gauss
Aug 5, 2004·Histochemistry and Cell Biology·William M Nauseef
Aug 24, 2004·Infection and Immunity·Jacob W IJdo, Angel C Mueller
Oct 19, 2004·Clinical Microbiology Reviews·Jennifer L Edwards, Michael A Apicella
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lee-Ann H AllenLara M Wittine
Mar 24, 2005·Infection and Immunity·Mark P SimonsMichael A Apicella
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jovanka M VoyichFrank R DeLeo
May 26, 2006·Nature Reviews. Immunology·Scott D Gray-Owen, Richard S Blumberg
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Kate L SeibAlastair G McEwan
Jun 17, 2006·The Journal of Clinical Investigation·Pham My-Chan DangJamel El-Benna
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takehiko UeyamaNaoaki Saito
Aug 8, 2008·Cellular Microbiology·Alison K Criss, H Steven Seifert

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Citations

May 15, 2015·Journal of Immunological Methods·Asya SmirnovAlison K Criss
Jan 22, 2015·The Journal of Infectious Diseases·Richard A JuneauAlison K Criss
May 2, 2015·Immunologic Research·Mark P Murphy, Emma Caraher
Aug 26, 2016·Immunological Reviews·Lauren C Kinkead, Lee-Ann H Allen
Sep 9, 2017·Cellular Microbiology·Anna SintsovaScott D Gray-Owen
Jan 30, 2018·Cellular Microbiology·Jacob S EdmissonSilvia M Uriarte
Apr 12, 2018·Journal of Innate Immunity·Scott D KobayashiFrank R DeLeo
Apr 4, 2017·Current Protocols in Cytometry·Asya SmirnovAlison K Criss
Aug 4, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shruti SrivastavaSangita Mukhopadhyay
Nov 5, 2019·Frontiers in Immunology·Michael W RussellScott D Gray-Owen
Sep 12, 2017·Frontiers in Cellular and Infection Microbiology·Giang T NguyenJoan Mecsas
Jan 12, 2019·Molecular Oral Microbiology·Melody Y ZengJuhi Bagaitkar

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