Assembly of photosystem I. I. Inactivation of the rubA gene encoding a membrane-associated rubredoxin in the cyanobacterium Synechococcus sp. PCC 7002 causes a loss of photosystem I activity

The Journal of Biological Chemistry
Gaozhong ShenD A Bryant

Abstract

A 4.4-kb HindIII fragment, encoding an unusual rubredoxin (denoted RubA), a homolog of the Synechocystis sp. PCC 6803 gene slr2034 and Arabidopsis thaliana HCF136, and the psbEFLJ operon, was cloned from the cyanobacterium Synechococcus sp. PCC 7002. Inactivation of the slr2034 homolog produced a mutant with no detectable phenotype and wild-type photosystem (PS) II levels. Inactivation of the rubA gene of Synechococcus sp. PCC 7002 produced a mutant unable to grow photoautotrophically. RubA and PS I electron transport activity were completely absent in the mutant, although PS II activity was approximately 80% of the wild-type level. RubA contains a domain of approximately 50 amino acids with very high similarity to the rubredoxins of anaerobic bacteria and archaea, but it also contains a region of about 50 amino acids that is predicted to form a flexible hinge and a transmembrane alpha-helix at its C terminus. Overproduction of the water-soluble rubredoxin domain in Escherichia coli led to a product with the absorption and EPR spectra of typical rubredoxins. RubA was present in thylakoid but not plasma membranes of cyanobacteria and in chloroplast thylakoids isolated from spinach and Chlamydomonas reinhardtii. Fractionation stu...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Mar 7, 1997·The Journal of Biological Chemistry·V V Bartsevich, H B Pakrasi
Jul 6, 2000·The Journal of Biological Chemistry·J WastlU G Maier
Sep 7, 2000·Protein Science : a Publication of the Protein Society·K SchweimerH Sticht
Apr 27, 2001·Nature·S DouglasU G Maier
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Parag R Chitnis
Jul 11, 2001·Biochemical and Biophysical Research Communications·E Guedon, H Petitdemange
Nov 1, 2001·Biochimica Et Biophysica Acta·I R VassilievJ H Golbeck
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S E Stevens, R D Porter
Mar 1, 1996·Photosynthesis Research·C G Lojero, D W Krogmann

❮ Previous
Next ❯

Citations

Oct 10, 2007·Photosynthesis Research·Marc BrechtRobert Bittl
Dec 7, 2006·Physical Chemistry Chemical Physics : PCCP·Oleg M SarkisovVictor A Nadtochenko
Nov 12, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Rebecca Ann GrimmeJohn Harvey Golbeck
Jan 17, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Sam MulaArt van der Est
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eva C M Nowack, Arthur R Grossman
Aug 1, 2013·The Journal of Biological Chemistry·Robert H CalderonKrishna K Niyogi
May 6, 2008·Molecular & Cellular Proteomics : MCP·Wojciech MajeranKlaas J van Wijk
Mar 2, 2010·Molecular & Cellular Proteomics : MCP·Mia TerashimaMichael Hippler
Nov 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Wei ChiLixin Zhang
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Carla Ayala-CastroF Wayne Outten
Jan 15, 2008·The Journal of Chemical Physics·M KrzystyniakMikhail L Antonkine
Dec 21, 2005·Biochimica Et Biophysica Acta·Ulf DühringAnnegret Wilde
Jan 15, 2015·Biochimica Et Biophysica Acta·Huixia YangCongming Lu
Feb 11, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Francesco BonomiAnna Morleo
Jul 11, 2014·The Journal of Biological Chemistry·Johnna L RooseTerry M Bricker
Dec 3, 2003·The Journal of Biological Chemistry·Jana Stöckel, Ralf Oelmüller
Sep 5, 2020·Critical Reviews in Biochemistry and Molecular Biology·Michael GorkaJohn H Golbeck
Apr 26, 2014·The Journal of Biological Chemistry·Shuyi ZhangDonald A Bryant
Jul 7, 2016·The Journal of Biological Chemistry·Yuehui ZhuHimadri B Pakrasi
Aug 22, 2006·The Journal of Biological Chemistry·Serena SchwenkertJörg Meurer

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.