Assembly of ROMK1 (Kir 1.1a) inward rectifier K+ channel subunits involves multiple interaction sites

Biophysical Journal
J C KosterK Ho

Abstract

The ROMK1 (Kir 1.1a) channel is formed by a tetrameric complex of subunits, each characterized by cytoplasmic N- and C-termini and a core region of two transmembrane helices flanking a pore-forming segment. To delineate the general regions mediating the assembly of ROMK1 subunits we constructed epitope-tagged N-terminal, C-terminal, and transmembrane segment deletion mutants. Nonfunctional subunits with N-terminal, core region, and C-terminal deletions had dominant negative effects when coexpressed with wild-type ROMK1 subunits in Xenopus oocytes. In contrast, coexpression of these nonfunctional subunits with Kv 2.1 (DRK1) did not suppress Kv 2.1 currents in control oocytes. Interactions between epitope-tagged mutant and wild-type ROMK1 subunits were studied in parallel by immunoprecipitating [35S]-labeled oocyte membrane proteins. Complexes containing both wild-type and mutant subunits that retained H5, M2, and C-terminal regions were coimmunoprecipitated to a greater extent than complexes consisting of wild-type and mutant subunits with core region and/or C-terminal deletions. The present findings are consistent with the hypothesis that multiple interaction sites located in the core region and cytoplasmic termini of ROMK1 sub...Continue Reading

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Citations

Jun 8, 2011·The Journal of Biological Chemistry·I-Hsiu ChenChung-Jiuan Jeng
Oct 26, 1999·The Journal of General Physiology·T P FlaggP A Welling
May 30, 2002·The Journal of General Physiology·Catherine A CukrasColin G Nichols
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Dec 25, 2004·Physiological Reviews·Steven C HebertWenhui Wang
Aug 21, 2013·Seminars in Nephrology·Paul A Welling
Dec 6, 2005·Protein Expression and Purification·Pranab K ChandaJeffrey D Kennedy
Sep 2, 2005·The Journal of Biological Chemistry·Heather M JonesDaniel C Devor
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Jul 31, 1999·The Journal of Biological Chemistry·J P GiblinA Tinker
May 29, 2003·American Journal of Physiology. Renal Physiology·James B BrunsThomas R Kleyman
Apr 6, 2000·American Journal of Physiology. Renal Physiology·M TanemotoJ Z Xu

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