Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs

Archives of Biochemistry and Biophysics
Timothy H BayburtStephen G Sligar

Abstract

Nanodiscs, phospholipid bilayer assemblies of controlled size, were used to self-assemble bacteriorhodopsin (bR) into single trimers. Self-assembly at optimal bR to Nanodisc and phospholipid stoichiometry yielded particles containing three bR molecules. Analysis of solution small angle X-ray scattering indicated that bacteriorhodopsin is embedded in a discoidal phospholipid bilayer structure. Formation of trimers, as evidenced by visible circular dichroism of the retinal absorbance bands, is facilitated in Nanodiscs at a specific size threshold, suggesting that a critical bilayer area or amount of lipid is necessary to maintain a native oligomeric state. The lipid to bR ratio in the assembly process was also found to be an important factor in determining oligomerization state. These nanoscale bilayers offer the opportunity to understand and control the assembly of oligomeric integral membrane proteins critical to macromolecular recognition and cellular signaling.

References

Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
May 6, 1998·Protein Science : a Publication of the Protein Society·E Wallin, G von Heijne
Aug 24, 1999·Journal of Molecular Biology·H LueckeJ K Lanyi
Nov 5, 1999·The Journal of Biological Chemistry·J P SegrestS C Harvey
Feb 2, 2000·Biophysical Journal·J García De La TorreB Carrasco
Sep 27, 2002·Biophysical Journal·Kamakshi JagannathanArun Yethiraj
Feb 15, 2003·Biochimica Et Biophysica Acta·M Opekarová, W Tanner
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Timothy H Bayburt, Stephen G Sligar
Mar 18, 2004·Journal of the American Chemical Society·I G DenisovS G Sligar
Sep 17, 2004·Archives of Biochemistry and Biophysics·Bradley J BaasStephen G Sligar
Dec 15, 2004·Biochemistry·Paul S-H ParkKrzysztof Palczewski
Jul 6, 2005·Biochemistry·Marc Chabre, Marc le Maire
Jul 21, 2006·The Journal of Physical Chemistry. B·Ilia G DenisovStephen G Sligar

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Citations

Feb 13, 2013·Analytical and Bioanalytical Chemistry·Michael T MartyStephen G Sligar
Sep 29, 2011·Journal of the American Chemical Society·Matthew J RanaghanRobert R Birge
Nov 18, 2009·Journal of the American Chemical Society·Thomas RaschleGerhard Wagner
Aug 2, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tomoko NakayamaDinah Wy Sah
Jul 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Thomas BoldogGerald L Hazelbauer
Jul 8, 2008·Molecular & Cellular Proteomics : MCP·Jenny A CappuccioMatthew A Coleman
Dec 3, 2010·Journal of Nanobiotechnology·Robert O Ryan
May 21, 2009·Biological Chemistry·Jonas Borch, Thomas Hamann
Sep 11, 2009·International Journal of Molecular Sciences·Craig D BlanchetteTodd A Sulchek
Feb 18, 2014·Acta Crystallographica. Section D, Biological Crystallography·Søren A R KyndeLise Arleth
Apr 18, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Tomohiro ImuraDai Kitamoto
Jun 6, 2014·Nature Protocols·Satoshi FujiiTetsuya Yomo
Nov 26, 2013·Advances in Colloid and Interface Science·Thomas Günther PomorskiMarité Cárdenas
Dec 22, 2015·Journal of the American Chemical Society·Sung Jun LimAndrew M Smith
Nov 27, 2015·The Journal of Physical Chemistry. B·Mikkel VestergaardBirgit Schiøtt
Jul 20, 2010·Experimental Neurology·Jian Rong ShengMatthew N Meriggioli
Aug 6, 2010·Biochimica Et Biophysica Acta·Ilia G Denisov, Stephen G Sligar
Jun 24, 2010·Current Opinion in Structural Biology·Thomas RaschleGerhard Wagner
Nov 18, 2015·Journal of Chemical Theory and Computation·Iwona Siuda, D Peter Tieleman
Mar 8, 2016·Biochimica Et Biophysica Acta·Sarah C LeeTimothy R Dafforn
Nov 5, 2015·Biophysical Journal·Tsung-Yen LeeTsyr-Yan Yu
Feb 1, 2011·Protein Science : a Publication of the Protein Society·Tingjuan GaoMatthew A Coleman
Jun 12, 2013·Protein Science : a Publication of the Protein Society·Wei HeMatthew A Coleman
Oct 31, 2006·Journal of Structural Biology·Amy Y ShihKlaus Schulten
Jun 27, 2013·Molecular Membrane Biology·Suzan Ruehrer, Hartmut Michel
Oct 25, 2014·Journal of Oleo Science·Tomohiro ImuraDai Kitamoto
May 6, 2008·Vision Research·Theodore G Wensel
Apr 21, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Wade F ZenoMarjorie L Longo
Feb 9, 2017·Chemical Reviews·Ilia G Denisov, Stephen G Sligar

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