Assembly of skeletal muscle cells on a Si-MEMS device and their generative force measurement

Biomedical Microdevices
Kazunori ShimizuEiji Nagamori

Abstract

We have fabricated a simple Si-MEMS device consisting of a microcantilever and a base to measure active tension generated by skeletal muscle myotubes derived from murine myoblast cell line C2C12. We have developed a fabrication process for integration of myotubes onto the device. To position myotubes over the gap between the cantilever and the base without damage due to mechanical peeling or the use of an enzymatic reaction, we cultured myotubes on poly-N-isopropylacrylamide (PNIPAAm) as a sacrifice layer. By means of immune staining of alpha-actinin, it was confirmed that a myotube micropatterned onto the device bridged the gap between the cantilever and the base. After 7d differentiation, the myotube was actuated by electrical stimulation. The active tension generated by the myotube was evaluated by measuring the bending of the cantilever using image processing. On twitch stimulation, the myotube on the device contracted and generated active tension in response to the electrical signals. On tetanus tension measurement, approximately 1.0 microN per single myotube was obtained. The device developed here can be used in wide area of in vitro skeletal muscle studies, such as drug screening, physiology, regenerative medicine, etc.

References

Jun 1, 1994·The American Journal of Physiology·D K McMahonN N Malouf
Jul 22, 1999·The New England Journal of Medicine·P R Shepherd, B B Kahn
Nov 25, 2000·Science·R K SoongC D Montemagno
Feb 24, 2001·American Journal of Physiology. Cell Physiology·R G DennisJ A Faulkner
Jul 18, 2001·Journal of Applied Physiology·T J Hawke, D J Garry
Sep 6, 2001·IEEE Transactions on Bio-medical Engineering·G LinK P Roos
Mar 6, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Satoshi NishimuraSeiryo Sugiura
Jan 18, 2005·Nature Materials·Jianzhong XiCarlo D Montemagno
Mar 3, 2006·Lab on a Chip·Yo TanakaTakehiko Kitamori
Sep 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuichi HiratsukaTaro Q P Uyeda
Apr 12, 2007·Experimental Cell Research·Hideaki FujitaMakoto Kanzaki
Sep 8, 2007·Science·Adam W FeinbergKevin Kit Parker
Feb 1, 2008·Muscle & Nerve·Herman VandenburghGregory Crawford
May 31, 2008·Journal of Tissue Engineering and Regenerative Medicine·Hyoungshin ParkGordana Vunjak-Novakovic

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Citations

Oct 20, 2010·Biomedical Microdevices·Hideaki FujitaEiji Nagamori
Jan 26, 2013·Journal of Bioscience and Bioengineering·Kazunori ShimizuSatoshi Konishi
Oct 3, 2012·Journal of Bioscience and Bioengineering·Kazunori ShimizuEiji Nagamori
Dec 10, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Rebecca M Duffy, Adam W Feinberg
Feb 24, 2010·Biotechnology and Bioengineering·Hideaki FujitaEiji Nagamori
Aug 3, 2014·Journal of Bioscience and Bioengineering·Kazunori ShimizuSatoshi Konishi
Jan 27, 2015·Annual Review of Pathology·Kambez H BenamDonald E Ingber
Sep 11, 2012·Biomedical Microdevices·Leonardo Ricotti, Arianna Menciassi
Aug 21, 2015·Bioinspiration & Biomimetics·L VannozziA Menciassi
May 18, 2020·Chemical Society Reviews·Lingyu SunYuanjin Zhao
Jun 11, 2020·Advanced Biosystems·Lorenzo VannozziLeonardo Ricotti
Dec 20, 2013·Advanced Healthcare Materials·Piyush BajajRashid Bashir
Nov 22, 2017·Science Robotics·Victoria A Webster-WoodRoger D Quinn
May 28, 2021·Science Robotics·Maria GuixSamuel Sánchez

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