PMID: 9557680Apr 29, 1998Paper

Assembly of the herpes simplex virus capsid: preformed triplexes bind to the nascent capsid

Journal of Virology
J V SpencerJ C Brown

Abstract

The herpes simplex virus type 1 (HSV-1) capsid is a T=16 icosahedral shell that forms in the nuclei of infected cells. Capsid assembly also occurs in vitro in reaction mixtures created from insect cell extracts containing recombinant baculovirus-expressed HSV-1 capsid proteins. During capsid formation, the major capsid protein, VP5, and the scaffolding protein, pre-VP22a, condense to form structures that are extended into procapsids by addition of the triplex proteins, VP19C and VP23. We investigated whether triplex proteins bind to the major capsid-scaffold protein complexes as separate polypeptides or as preformed triplexes. Assembly products from reactions lacking one triplex protein were immunoprecipitated and examined for the presence of the other. The results showed that neither triplex protein bound unless both were present, suggesting that interaction between VP19C and VP23 is required before either protein can participate in the assembly process. Sucrose density gradient analysis was employed to determine the sedimentation coefficients of VP19C, VP23, and VP19C-VP23 complexes. The results showed that the two proteins formed a complex with a sedimentation coefficient of 7.2S, a value that is consistent with formation of...Continue Reading

References

May 1, 1978·Journal of Virology·J C D'HalluinP A Boulanger
Aug 1, 1976·Journal of Virology·B EdvardssonL Philipson
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B L TrusA C Steven
Feb 24, 1989·Cell·J D SchragW Chiu
Jul 1, 1988·The Journal of General Virology·D J McGeochP Taylor
Aug 4, 1995·Journal of Molecular Biology·O J MarvikB H Lindqvist
Sep 1, 1994·Journal of Virology·W W NewcombJ C Brown
May 1, 1994·The Journal of General Virology·J D TatmanF J Rixon
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·F P BooyA C Steven
Sep 26, 1997·Nature·T DoklandM G Rossmann

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Citations

Jul 5, 2005·Journal of Virology·Xiaodong YanTimothy S Baker
Jan 18, 2006·Journal of Virology·Walt E AdamsonFrazer J Rixon
Dec 15, 2010·Journal of Virology·Wen-Hung WangShih-Tung Liu
May 16, 2014·The Journal of General Virology·Christopher M CapuanoPrashant J Desai
Jun 19, 2015·Journal of Virology·Wen-Hung WangShih-Tung Liu
Dec 28, 2005·Journal of Virology·Mercy E OkoyePrashant Desai
Apr 26, 2016·Nature Structural & Molecular Biology·Alexis HuetJames F Conway
Sep 4, 2001·Biotechnology & Genetic Engineering Reviews·J I Casal
Jan 23, 2009·Journal of Virology·Brandon W HensonPrashant Desai
Oct 8, 2015·MBio·Anastasia A AksyukAlasdair C Steven
Aug 2, 2017·The Journal of General Virology·Martin Zühlsdorf, Winfried Hinrichs
Nov 6, 2018·Frontiers in Microbiology·Francisco J IbáñezPablo A González
Feb 16, 2021·The Journal of the Korean Physical Society·Joo-Hyeon LeeSeung Joong Kim
Nov 13, 1998·Journal of Virology·M D KirkitadzeD A McClelland
Aug 10, 2000·Neoplasia : an International Journal for Oncology Research·A JacobsC Fraefel
Jun 2, 2007·Journal of Molecular Biology·William W NewcombJay C Brown

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