PMID: 375006Jan 31, 1979Paper

Assembly of the mitochondrial membrane system: two separate genes coding for threonyl-tRNA in the mitochondrial DNA of Saccharomyces cerevisiae

Molecular & General Genetics : MGG
G Macino, A Tzagoloff

Abstract

1. Mitochondria of Saccharomyces cerevisiae contain two tRNA's that are acylated with threonine. The two isoaccepting species (tRNA1Thr and tRNA2Thr) can be separated by reversed-phase chromatography on RPC-5. 2. A cytoplasmic mutant has been isolated which lacks tRNA1Thr but has normal levels of tRNA2Thr. This mutation was previously shown to map between the oxi 1 and oxi 2 loci on mitochondrial DNA. 3. tRNA1Thr and tRNA2Thr hybridize to wild type mitochondrial but not nuclear DNA and are capable of partially competing with each other. Hybridization of each species to different segments of mitochondrial DNA isolated from p- clones indicate that there are two threonyl tRNA genes. One gene is located between oxi 1 and oxi 2 and codes for tRNA1Thr. The second gene codes for tRNA2Thr and is near the cap locus. 4. Binding assays to E. coli ribosomes indicate that tRNA2Thr recognizes the threonine triplet ACA and may also recognize the other three triplets but with a much lower efficiency. None of the four codons for threonine stimulate the binding of tRNA1Thr to the ribosomes.

References

Apr 23, 1976·Molecular & General Genetics : MGG·H Fukuhara, M Bolotin-Fukuhara
Nov 25, 1975·Journal of Molecular Biology·N ChiuY Suyama
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·W T Hsu, S B Weiss
Feb 28, 1970·Journal of Molecular Biology·G BernardiP P Slonimski
Jan 31, 1963·Biochemical and Biophysical Research Communications·R W HOLLEY

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Citations

Feb 1, 1989·European Journal of Biochemistry·A TzagoloffA Akai
Aug 1, 1979·Molecular & General Genetics : MGG·E CollettiH Fukuhara
Apr 5, 1991·Journal of Molecular Biology·D L Gatti, A Tzagoloff

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