PMID: 8567730Jan 1, 1996Paper

Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA

The Journal of Cell Biology
C Macaulay, D J Forbes


A key event in nuclear formation is the assembly of functional nuclear pores. We have used a nuclear reconstitution system derived from Xenopus eggs to examine the process of nuclear pore assembly in vitro. With this system, we have identified three reagents which interfere with nuclear pore assembly, NEM, GTP gamma S, and the Ca++ chelator, BAPTA. These reagents have allowed us to determine that the assembly of a nuclear pore requires the prior assembly of a double nuclear membrane. Inhibition of nuclear vesicle fusion by pretreatment of the membrane vesicle fraction with NEM blocks pore complex assembly. In contrast, NEM treatment of already fused double nuclear membranes does not block pore assembly. This indicates that NEM inhibits a single step in pore assembly--the initial fusion of vesicles required to form a double nuclear membrane. The presence of GTP gamma S blocks pore assembly at two distinct steps, first by preventing fusion between nuclear vesicles, and second by blocking a step in pore assembly that occurs on already fused double nuclear membranes. Interestingly, when the Ca2+ chelator BAPTA is added to a nuclear assembly reaction, it only transiently blocks nuclear vesicle fusion, but completely blocks nuclear p...Continue Reading


Jan 1, 1992·Annual Review of Cell Biology·D J Forbes
Aug 1, 1992·Current Opinion in Cell Biology·L Gerace
Jan 1, 1992·The Journal of Cell Biology·A L BomanK L Wilson
Jan 1, 1991·Biology of the Cell·M C Dabauvalle, U Scheer
Dec 1, 1990·The Journal of Cell Biology·J W NewportW G Dunphy
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M K ParkJ A Hanover
Jun 1, 1989·The Journal of Cell Biology·R W WozniakG Blobel
Feb 1, 1987·The Journal of Cell Biology·D R FinlayD J Forbes
Dec 1, 1982·The Journal of Cell Biology·L GeraceC Kondor-Koch
Apr 1, 1995·Trends in Biochemical Sciences·A L Boman, R A Kahn
Mar 1, 1995·Current Biology : CB·R B Kelly
Jan 6, 1995·The Journal of Biological Chemistry·C MacaulayD J Forbes
Jun 1, 1994·Current Opinion in Cell Biology·E Fabre, E C Hurt
Nov 3, 1994·Nature·J E Rothman
Dec 1, 1993·The Journal of Cell Biology·V C CordesW W Franke
Jan 1, 1993·Annual Review of Biochemistry·M A Osborne, P A Silver
Jul 1, 1993·The Journal of Cell Biology·N Chaudhary, J C Courvalin

❮ Previous
Next ❯


May 10, 2000·Current Opinion in Cell Biology·K J Ryan, S R Wente
May 10, 2001·Current Opinion in Cell Biology·S K Vasu, D J Forbes
Aug 1, 1996·Current Opinion in Cell Biology·C Denesvre, V Malhotra
Jun 1, 1997·Current Opinion in Cell Biology·V Doye, E Hurt
Oct 3, 2007·Nature Cell Biology·Brian Burke
Feb 24, 2009·Nature Reviews. Molecular Cell Biology·Stephan GüttingerUlrike Kutay
Nov 24, 1999·The Biochemical Journal·M MichalakM Opas
Oct 24, 1997·Development, Growth & Differentiation·D Poccia, P Collas
Aug 22, 2009·The Journal of Biological Chemistry·Elvira R RafikovaLeonid V Chernomordik
Oct 11, 2003·Molecular Biology of the Cell·Amnon HarelDouglass J Forbes
Dec 22, 2006·Molecular Biology of the Cell·Kathryn J RyanSusan R Wente
Jul 4, 2008·Molecular Biology of the Cell·Beth A RasalaDouglass J Forbes
Jul 25, 2009·Molecular Biology of the Cell·Asaf RotemAmnon Harel
Jul 31, 2009·Molecular Biology of the Cell·Corine K LauDouglass J Forbes
Oct 12, 2010·Molecular Biology of the Cell·Boris FichtmanDouglass J Forbes
Feb 4, 2011·Molecular Biology of the Cell·Anca F SavulescuAmnon Harel
Jul 8, 2008·Human Reproduction Update·Jason E Swain, Thomas B Pool
Jan 4, 2008·Molecular and Cellular Biology·Karen K ResendesDouglass J Forbes
Nov 5, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·K BodoorB Burke
May 7, 2009·Annual Review of Biophysics·Banafshé Larijani, Dominic L Poccia
Jan 1, 1997·Annual Review of Cell and Developmental Biology·T M Gant, K L Wilson
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Martin W HetzerIain W Mattaj
Mar 28, 2008·BMC Cell Biology·Valerie A DelmarDouglass J Forbes
Jan 23, 2014·Mechanisms of Ageing and Development·Boris Fichtman, Amnon Harel
Jan 31, 2014·Molecular Biology of the Cell·Cyril BernisDouglass J Forbes
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sharon Wald KraussRebecca Heald
Mar 11, 2009·The Journal of Cell Biology·T Renee DawsonSusan R Wente
May 17, 2006·The Journal of Cell Biology·Fabrizia StavruDirk Görlich
Mar 26, 2003·The Journal of Cell Biology·Kathryn J RyanSusan R Wente
Jun 1, 2006·The Journal of Cell Biology·Lori L O'Brien, Christiane Wiese
Jul 13, 2001·The Journal of Cell Biology·R I Lopez-SolerR D Goldman
Mar 24, 1997·The Journal of Cell Biology·M Bucci, S R Wente

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.