Assembly of the Trypanosoma brucei 60S ribosomal subunit nuclear export complex requires trypanosome-specific proteins P34 and P37.

Eukaryotic Cell
Kimberly Prohaska, Noreen Williams

Abstract

We previously identified two Trypanosoma brucei RNA binding proteins, P34 and P37, and determined that they are essential for proper ribosomal assembly in this organism. Loss of these proteins via RNA interference is lethal and causes a decrease in both 5S rRNA levels and formation of 80S ribosomes, concomitant with a decrease in total cellular protein synthesis. These data suggest that these proteins are involved at some point in the ribosomal biogenesis pathway. In the current study, we have performed subcellular fractionation in conjunction with immune capture experiments specific for 60S ribosomal proteins and accessory factors in order to determine when and where P34 and P37 are involved in the ribosomal biogenesis pathway. These studies demonstrate that P34 and P37 associate with the 60S ribosomal subunit at the stage of the nucleolar 90S particle and remain associated subsequent to nuclear export. In addition, P34 and P37 associate with conserved 60S ribosomal subunit nuclear export factors exportin 1 and Nmd3, suggesting that they are components of the 60S ribosomal subunit nuclear export complex in T. brucei. Most significantly, the pre-60S complex does not associate with exportin 1 or Nmd3 in the absence of P34 and P3...Continue Reading

References

Jun 1, 1990·Molecular and Biochemical Parasitology·S Michaeli, N Agabian
Feb 1, 1987·The Journal of protozoology·S Z Shapiro, B E Kimmel
Sep 1, 1997·Molecular and Cellular Biology·D P EisingerB L Trumpower
May 9, 1998·Molecular and Biochemical Parasitology·J ZhangN Williams
Jan 8, 1999·Molecular and Biochemical Parasitology·M Brecht, M Parsons
Jun 29, 1999·The Journal of Cell Biology·A M DechampesmeS Camier
Nov 5, 1999·Trends in Biochemical Sciences·J R Warner
Nov 24, 1999·Molecular and Cellular Biology·D KresslerJ de La Cruz
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·T V PestovaC U Hellen
Jan 10, 2002·Biochemical and Biophysical Research Communications·Joseph PitulaNoreen Williams
Jun 18, 2002·Current Opinion in Cell Biology·Alessandro Fatica, David Tollervey
Jun 22, 2002·Molecular and Biochemical Parasitology·Joseph S PitulaNoreen Williams
Apr 10, 2003·Eukaryotic Cell·Gusti M ZeinerDavid A Campbell
May 30, 2003·The EMBO Journal·Christopher R TrottaJames E Dahlberg
Jun 6, 2003·The Journal of Biological Chemistry·Bryan C JensenMarilyn Parsons
Jun 17, 2003·Molecular and Cellular Biology·Cosmin SaveanuMicheline Fromont-Racine
Sep 6, 2003·Gene·Micheline Fromont-RacineFranco Fasiolo
Feb 18, 2004·The Journal of Clinical Investigation·Peter G E Kennedy
Jun 11, 2004·Annual Review of Biochemistry·Lee D Kapp, Jon R Lorsch
Oct 26, 2005·Molecular Cell·Sébastien Ferreira-CercaPhilipp Milkereit
May 3, 2006·The Journal of Cell Biology·Alice LebretonMicheline Fromont-Racine
Apr 6, 2007·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Vincent Delespaux, Harry P de Koning
Jul 10, 2007·British Journal of Pharmacology·M P BarrettR R Tidwell
Nov 13, 2007·Molecular Cell·Sébastien Ferreira-CercaPhilipp Milkereit
Dec 14, 2007·Molecular Biology of the Cell·Nai-Jung HungArlen W Johnson

❮ Previous
Next ❯

Citations

Jan 4, 2013·The Journal of Biological Chemistry·Sachin Kumar GuptaShulamit Michaeli
Jun 27, 2012·Current Opinion in Pediatrics·Daniel Cuevas-RamosFrancisco J Gómez-Pérez
Feb 15, 2013·PLoS Neglected Tropical Diseases·María de los Milagros CámaraClaudio A Pereira
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Christine Clayton, Shula Michaeli
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Martin Ciganda, Noreen Williams
Apr 16, 2015·Nucleic Acids Research·Melanie BühlmannKeith R Matthews
Mar 17, 2016·World Journal of Biological Chemistry·María Albertina RomaniukAlejandro Cassola
Jul 10, 2018·The Journal of Eukaryotic Microbiology·Helisa H WippelLysangela R Alves
May 11, 2019·Cells·Santiago Martínez-CalvilloTomás Nepomuceno-Mejía
Jan 4, 2019·Nucleic Acids Research·Vaibhav ChikneShulamit Michaeli
Aug 9, 2020·The Journal of Biological Chemistry·Kathrin BajakEsteban Erben

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.