PMID: 9660861Jul 14, 1998Paper

Assembly of the yeast vacuolar H+-ATPase occurs in the endoplasmic reticulum and requires a Vma12p/Vma22p assembly complex

The Journal of Cell Biology
Laurie A GrahamT H Stevens

Abstract

Three previously identified genes from Saccharomyces cerevisiae, VMA12, VMA21, and VMA22, encode proteins localized to the endoplasmic reticulum (ER). These three proteins are required for the biogenesis of a functional vacuolar ATPase (V-ATPase), but are not part of the final enzyme complex. Subcellular fractionation and chemical cross-linking studies have revealed that Vma12p and Vma22p form a stable membrane associated complex. Cross-linking analysis also revealed a direct physical interaction between the Vma12p/Vma22p assembly complex and Vph1p, the 100-kD integral membrane subunit of the V-ATPase. The interaction of the Vma12p/Vma22p complex with Vph1p was transient (half-life of approximately 5 min), reflecting trafficking of this V-ATPase subunit through the ER en route to the vacuolar membrane. Analysis of these protein-protein interactions in ER-blocked sec12 mutant cells indicated that the Vph1p-Vma12p/Vma22p interactions are quite stable when transport of the V-ATPase out of the ER is blocked. Fractionation of solubilized membrane proteins on a density gradient revealed comigration of Vma22p and Vma12p, indicating that they form a complex even in the absence of cross-linker. Vma12p and Vma22p migrated to fractions se...Continue Reading

References

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Citations

Nov 15, 2007·Journal of Bioenergetics and Biomembranes·Patricia M Kane
Sep 3, 1999·Molecular Biology of the Cell·M HorstP V Schu
Apr 11, 2003·Molecular Biology of the Cell·April S GoehringGeorge F Sprague
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Feb 3, 2006·Molecular Biology of the Cell·Nicole R QuennevilleElizabeth Conibear
Nov 17, 2006·Molecular Biology of the Cell·Margaret M Kincaid, Antony A Cooper
Sep 19, 2008·Molecular Biology of the Cell·Margret RyanTom H Stevens
Sep 9, 2006·Journal of Gastroenterology and Hepatology·Fred S GorelickEdwin C Thrower
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Patricia M Kane
May 30, 2008·BMC Cell Biology·Thorsten SeidelKarl-Josef Dietz
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May 2, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Quan-Sheng Qiu
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Feb 24, 2018·Traffic·Laurie A GrahamPatricia M Kane
Jul 20, 2017·Molecular Biology of the Cell·Subhrajit Banerjee, Patricia M Kane

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