PMID: 2121744Nov 1, 1990Paper

Assembly properties of dominant and recessive mutations in the small mouse neurofilament (NF-L) subunit

The Journal of Cell Biology
S R GillD W Cleveland

Abstract

We have generated a set of amino- and carboxy-terminal deletions of the NF-L neurofilament gene and determined the assembly properties of the encoded subunits after coexpression with vimentin or wild-type NF-L. NF-L molecules missing greater than 30% (31 amino acids of the head) or 90% (128 amino acids of the tail) failed to incorporate into intermediate filament networks. Carboxy-terminal deletions into the rod domain yield dominant mutants that disrupt arrays assembled from wild-type subunits, even when present at levels of approximately 2% of the wild-type subunits. Even mutants retaining 55% of the tail (61 amino acids) disrupt normal arrays when accumulated above approximately 10% of wild-type subunits. Since deletion of greater than 90% of the head domain produces "recessive" assembly incompetent subunits that do not affect wild-type filament arrays, whereas smaller deletions yield efficient network disruption, we conclude that some sequence(s) in the head domain (within residues 31-87) are required for the earliest steps in filament assembly. Insertional mutagenesis in the nonhelical spacer region within the rod domain reveals that as many as eight additional amino acids can be tolerated without disrupting assembly compe...Continue Reading

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Citations

Feb 1, 1997·Journal of Neuroscience Research·L A WhiteS R Whittemore
Sep 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R L Shoeman, P Traub
Jan 1, 1992·Cell Motility and the Cytoskeleton·R A Nixon, T B Shea
Jan 1, 1995·Cell Motility and the Cytoskeleton·J M Andreoli, K T Trevor
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May 18, 2005·Journal of the Neurological Sciences·Axel Petzold
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Feb 1, 1993·Current Opinion in Cell Biology·P A Coulombe
Jun 19, 1998·Current Opinion in Structural Biology·H Herrmann, U Aebi
Jul 1, 1997·The International Journal of Biochemistry & Cell Biology·A WaseemN H Waseem
Feb 14, 2002·Chemical Reviews·Natasha E Zachara, Gerald W Hart
Oct 11, 2011·Biologie aujourd'hui·Pierre JoanneOnnik Agbulut
Sep 1, 1991·The Journal of Cell Biology·T SchultheissH Holtzer
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Apr 1, 1993·The Journal of Cell Biology·O OharaT Kitamura
Sep 1, 1993·The Journal of Cell Biology·M K LeeD W Cleveland
May 1, 1994·The Journal of Cell Biology·E Fuchs
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Feb 15, 2000·Annals of the New York Academy of Sciences·Y NagaiJ R Burke

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